Standardized molecular diagnostic testing for vitreoretinal lymphoma

DECODE VRL - Multicentric Implementation and Clinical Validation of Standardized, Innovative Molecular Diagnostics for VitreoRetinal Lymphoma

Observational University of Luebeck · NCT05841914

This project will test whether centralized molecular testing of eye and blood samples can help doctors diagnose and monitor adults with suspected vitreoretinal lymphoma.

Quick facts

Study typeObservational
Enrollment220 (estimated)
Ages18 Years and up
SexAll
SponsorUniversity of Luebeck Academic / other
Drugs / interventionschemotherapy
Locations1 site (Lübeck, Schleswig-Holstein)
Trial IDNCT05841914 on ClinicalTrials.gov

What this trial studies

This prospective, multicentre observational project will collect aqueous, vitreous, blood serum and occasional retinal/choroidal samples from at least 220 adults with suspected vitreoretinal lymphoma. Diagnostic samples will be processed centrally using a standardized pipeline and subjected to multiparametric molecular analyses including next-generation sequencing for mutations and clonality detection and miRNA profiling. Patients will be followed for up to 24 months to link molecular biomarkers with diagnostic outcomes and treatment response. The aim is to determine whether standardized molecular diagnostics improve diagnostic sensitivity and provide useful monitoring biomarkers compared with current standard work-up.

Who should consider this trial

Good fit: Adults (18+) with clinical suspicion of vitreoretinal lymphoma who are scheduled for diagnostic vitrectomy and can provide informed consent are eligible for inclusion.

Not a fit: Patients who recently received systemic chemotherapy (within 3 months), recent systemic corticosteroids (within 2 weeks), who cannot undergo vitrectomy, or whose disease is ultimately not VRL may not benefit from the molecular testing.

Why it matters

Potential benefit: If successful, this approach could lead to faster and more accurate diagnoses and give clinicians better tools to monitor treatment response in vitreoretinal lymphoma.

How similar studies have performed: Molecular methods such as NGS, clonality testing, and miRNA profiling have shown promise in retrospective series, but prospective multicentre validation in VRL is limited.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Clinical suspicion of the presence of VRL with indication for diagnostic vitrectomy. Clinical suspicion is based on the assessment of the treating physicians in the clinics participating in this study.
* Age over 18 years.
* Written informed consent to participate in the study.

Exclusion Criteria:

* Systemic chemotherapy for lymphoma within 3 months prior to vitrectomy.
* Cortisone use within 2 weeks before vitrectomy, macular oedema, posterior synechiae, rubeosis iridis

Where this trial is running

Lübeck, Schleswig-Holstein

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Vitreoretinal LymphomaVitreoretinal lymphomaprimary CNS lymphomaimproved detectionmolecular diagnosticsdiagnostic standardisationhealth services researchprospective study
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.