Single-dose test of CITY-FXI in adults with and without Factor V Leiden or Prothrombin G20210A mutation

A Phase 1, Randomised, Double-Blind, Placebo-Controlled Single Ascending Dose Study to Evaluate the Safety, Tolerability, Pharmacokinetics, and Pharmacodynamics of CITY-FXI, a FXI Targeting siRNA, Administered Subcutaneously in Healthy Adults and Adults With Factor V Leiden or Prothrombin G20210A Mutation

Phase 1 Interventional City Therapeutics · NCT07430397

This will test a single dose of CITY-FXI, an siRNA that lowers Factor XI, in healthy adults and in adults who carry Factor V Leiden or the prothrombin G20210A mutation to see if it is safe and how it acts in the body.

Quick facts

PhasePhase 1
Study typeInterventional
Enrollment128 (estimated)
Ages18 Years to 60 Years
SexAll
SponsorCity Therapeutics Industry-sponsored
Locations1 site (London)
Trial IDNCT07430397 on ClinicalTrials.gov

What this trial studies

This is a first-in-human, randomized, double-blind, placebo-controlled single ascending dose trial conducted at one center. It includes two parts: ascending single doses in healthy volunteers and ascending single doses in adults with confirmed Factor V Leiden or prothrombin G20210A mutation. The main goals are to characterize safety, tolerability, pharmacokinetics, and pharmacodynamics after a single administration of CITY-FXI compared with placebo. Participants are monitored for adverse events, blood markers of coagulation, and drug levels over a defined follow-up period.

Who should consider this trial

Good fit: Ideal participants are adults (Part A: ages 18–45 non-childbearing potential; Part B: ages 18–60) with BMI 18–25 kg/m2 who can comply with study procedures, and Part B participants must have genetically confirmed Factor V Leiden or prothrombin G20210A mutation.

Not a fit: Patients with active bleeding disorders, a history of clinically significant spontaneous bleeding, homozygous or combined thrombophilic defects, significant systemic diseases, pregnant or breastfeeding individuals, or those outside the age/weight/BMI criteria are unlikely to qualify or benefit from this early single-dose trial.

Why it matters

Potential benefit: If successful, CITY-FXI could offer a new way to reduce clotting risk by lowering Factor XI activity, potentially with less bleeding than current anticoagulants.

How similar studies have performed: Previous clinical programs targeting Factor XI (using antisense oligonucleotides, antibodies, and other inhibitors) have shown promising reductions in thrombosis with relatively low bleeding rates, but CITY-FXI as an siRNA is being tested in humans for the first time.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Males and women of non-childbearing potential (WONCBP) aged 18 to 45 years (Part A only)
* Male and female participants aged 18 to 60 (Part B only)
* Body Mass Index (BMI) between 18 and 25 kg/m2 (inclusive) and a minimum weight of 50 kg
* Ability and willingness to comply fully with all study procedures and lifestyle considerations
* Confirmed diagnosis of FVL or prothrombin G20210A mutation via genetic testing (Part B only)
* Women of childbearing potential (WOCBP) must agree to use acceptable highly effective contraceptive methods (Part B only)

Exclusion Criteria:

* Any clinically significant systemic disease or disorder, including but not limited to cardiovascular, hepatic, or oncological conditions
* History or evidence of any bleeding disorders
* History of clinically significant spontaneous bleeding
* Prior treatment with an investigational agent
* Confirmed diagnosis of homozygous mutations, or combined thrombophilic defects of (Part B only)

Where this trial is running

London

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Factor V LeidenProthrombin G20210AFactor XIsiRNA
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.