Single-cell and spatial mapping of cells in gum tissue from aggressive versus chronic periodontitis

Is this trial a fit for you?

A plain-English reading of the study's rules. Only the study team can confirm whether you qualify.

What this trial studies

Researchers will collect gingival tissue from patients already scheduled for tooth extraction or periodontal surgery and from healthy control donors, then profile those samples with single-cell and spatial transcriptomics to map cell types, states, and their tissue locations. Peripheral blood will undergo whole-genome sequencing to search for disease-linked genetic variants associated with aggressive periodontitis. The study compares cellular composition, mesenchymal stem cell subpopulations, gene expression signatures, signaling pathways, and spatial microenvironment patterns across aggressive, chronic, and healthy tissues. Data analysis will highlight molecular markers and spatial features that distinguish aggressive periodontitis from more common chronic forms.

Why it matters

Who can join: the full rules

Inclusion Criteria:

Aggressive Periodontitis Patients:

1. Aged 20-35 years
2. Radiographic evidence of at least 30% vertical bone loss at ≥2 tooth sites
3. Can be classified as Stage III/IV, Grade C periodontitis according to the 2018 new classification of periodontitis
4. With or without family history of periodontitis
5. No significant local contributing factors (e.g., calculus, occlusal trauma, etc.)

Chronic Periodontitis Patients:

1. Aged 30-40 years
2. ≥3 sites with probing depth ≥5 mm
3. Can be classified as Stage I/II, Grade A/B periodontitis according to the 2018 new classification of periodontitis
4. No significant family history
5. Disease predominantly driven by local factors (dental plaque, calculus, etc.)

Healthy Controls:

1. Aged 20-40 years
2. Radiographic examination shows no significant alveolar bone loss
3. Clinically cannot be diagnosed with gingivitis or periodontitis

Exclusion Criteria:

1. Use of antibiotics within the past 3 months
2. Smoking \>10 cigarettes per day
3. Uncontrolled severe diabetes (HbA1c level ≥6.7%)
4. Planning pregnancy, currently pregnant, or breastfeeding
5. Alcohol or drug addiction
6. Long-term use of immunosuppressive agents
7. Infectious diseases such as hepatitis or AIDS/HIV
8. History of malignant tumor, radiotherapy, or chemotherapy within the past 5 years

Where it's running

• 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China
  Hangzhou, Zhejiang, China Recruiting

Study contacts

• Study coordinator: Li-li Zhou
• Email: sophiazhou04@163.com
• Phone: +86 18329193003

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How to take part

1. Review the eligibility criteria above with your treating physician.
2. Check the official listing on ClinicalTrials.gov for the latest contact details and recruitment status.
3. Contact the study coordinator or principal investigator to ask for pre-screening. Pre-screening is free and never obligates you to enroll.