Sequential treatment for relapsed B-ALL using targeted CAR T-cells

Sequential Treatment With CD20/CD22/CD10-CART After CD19-CART Treatment Base on MRD in Relapsed/Refractory B-ALL

Early Phase 1 Interventional Zhujiang Hospital · NCT03407859

This study is testing a new treatment for patients with relapsed B-cell leukemia to see if using different CAR T-cell therapies can help them stay in remission longer.

Quick facts

PhaseEarly Phase 1
Study typeInterventional
Enrollment100 (estimated)
Ages18 Years to 60 Years
SexAll
SponsorZhujiang Hospital Academic / other
Drugs / interventionschemotherapy, CAR T, CART, CAR-T, Chimeric Antigen Receptor, immunotherapy
Locations1 site (Guangdong, Guangdong)
Trial IDNCT03407859 on ClinicalTrials.gov

What this trial studies

This clinical trial investigates a sequential treatment approach for patients with relapsed or refractory B-cell acute lymphoblastic leukemia (B-ALL) who have not achieved complete remission after standard chemotherapy. The study utilizes a combination of CAR T-cell therapies targeting CD20, CD22, and CD10 after initial treatment with CD19 CAR T-cells. The goal is to eliminate CD19-negative B-ALL cells that may cause relapse and to prolong remission times. The trial focuses on patients with specific cell phenotypes and aims to improve outcomes for those who have limited treatment options.

Who should consider this trial

Good fit: Ideal candidates include patients with relapsed or refractory B-ALL who have not responded to standard chemotherapy and have specific CD antigen expressions.

Not a fit: Patients with CD19-positive minimal residual disease or those with severe comorbidities may not benefit from this study.

Why it matters

Potential benefit: If successful, this treatment could significantly extend remission periods for patients with relapsed B-ALL.

How similar studies have performed: While CAR T-cell therapies have shown promise in treating B-ALL, this specific sequential approach targeting multiple antigens is relatively novel and has not been extensively tested.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1. Relapsed/Refractory B-ALL patients
2. Did not achieve complete remission after 2 times of standard plan chemotherapy
3. Relapsed after first induction chemotherapy
4. Did not response to chemotherapy before HSCT or relapsed after HSCT
5. Cannot receive allo-HSCT or refuse to receive allo-HSCT
6. Cell phenotype is CD19 and CD20/CD22/CD10/CD70 positive (single or combined)
7. Estimated survival time is more than 3 months in leukemia
8. Volunteered for this clinical trail and signed a consent form

Exclusion Criteria:

1. MRD was negative while the cell phenotype was CD19 expressed
2. Patients with severe insufficient cardiac, pulmonary and hepatorenal functions
3. Patients with severe mental illness, neurological disease or infectious disease
4. Patients with GVHD was taking immunosuppressants
5. Pregnant or lactating women
6. Patients have received other genetic therapy products
7. Transfection efficiency was less than 30%
8. Any situation may do harm to the subjects or interfere the results

Where this trial is running

Guangdong, Guangdong

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Therapy Related LeukemiaCD19-CARTCD20-CARTCD10-CARTCD22-CARTRelapsed/Refractory B-ALLMRDCD70-CART
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.