rFSH versus hMG effects on cumulus cell telomere length and blastocyst development

Does Recombinant FSH (rFSH, i.g. Gonal F®) as Compared to Human Menopausal Gonadotrophin (hMG) Affect Telomere Length of Cumulus Cells During Antral Follicle Growth and Impact Blastocyst Status?

Observational ART Fertility Clinics LLC · NCT06732843

This trial will test whether using recombinant FSH (Gonal‑F) versus human menopausal gonadotropin (Menopur) during ovarian stimulation changes cumulus cell telomere length and later blastocyst outcomes in women 18–38 undergoing IVF with PGT‑A.

Quick facts

Study typeObservational
Enrollment10 (estimated)
Ages18 Years to 38 Years
SexFemale
SponsorART Fertility Clinics LLC Academic / other
Locations1 site (Abu Dhabi, Abu Dhabi Emirate)
Trial IDNCT06732843 on ClinicalTrials.gov

What this trial studies

This is a randomized, open‑label, cross‑over study in expected normo‑responders comparing stimulation with rFSH (Gonal‑F) and HP‑hMG (Menopur). Each participant will undergo two stimulation cycles with a washout period, and cumulus cells will be collected to measure telomere length alongside standard embryo quality and hormonal markers. The trial builds on prior MEGASET findings showing comparable ongoing pregnancy rates but differing early pregnancy loss between the products. Outcomes will include telomere length in cumulus cells, blastocyst development metrics, and associated hormonal profiles.

Who should consider this trial

Good fit: Women aged 18–38 with BMI 18–30, expected normo‑responders (AFC 5–10 per ovary), requesting PGT‑A, with normal karyotypes and willing to undergo two stimulations and clinic visits in Abu Dhabi.

Not a fit: Patients who are poor responders, have severe endometriosis, recent hormonal pretreatment, recurrent miscarriages, abnormal karyotypes, or who do not want two stimulation cycles or PGT‑A are unlikely to benefit from this protocol.

Why it matters

Potential benefit: If successful, the findings could help clinicians choose the gonadotropin that better preserves oocyte/embryo quality and potentially reduce early pregnancy loss.

How similar studies have performed: Previous MEGASET and MEGASET‑HR trials showed similar ongoing pregnancy rates between Menopur and Gonal‑F and lower early pregnancy loss with HP‑hMG, while telomere length has been linked to oocyte quality, but direct head‑to‑head telomere comparisons are novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1. Maternal age 18 - 38 years
2. Patients requested PGT-A
3. BMI 18- 30kg/m2
4. Expected normoresponders
5. Fresh autologous ejaculate (≥5 mill/ml)
6. Sperm abstinence: 2-3 days
7. Normal female/male karyotype
8. Regular menstrual cycle length: 25-32 days
9. AFC of 5-10 in each ovary at the beginning of the stimulation
10. Trigger: dual trigger: 2.500 IU urinary hCG + 0.3 mg Decapeptyl. (13)
11. A wash-out period between 1 to 3 months; an inter-cycle variation of 10% in AFC, measured by the same physician, is accepted.
12. Couple agrees to perform two stimulations before continuing with embryo transfer

Exclusion Criteria:

* 1\. Uterine abnormalities as diagnosed by ultrasound 2. Any hormonal or oral contraceptive pretreatment of 3 months preceding the treatment 3. Endometriosis according to American Fertility Society (AFS) ≥3 4. ≥ 2 miscarriages 5. Bologna criteria poor responders (14)

Where this trial is running

Abu Dhabi, Abu Dhabi Emirate

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions OVARIAN STIMULATIONTelomere LengthTelomere Length, Mean Leukocyterecombinant human follicle-stimulating hormoneuman-menopausal-gonadotropin (hMGocyte qualityembryo qualityblastocyst development
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.