Repeated neural stem cell–delivered virotherapy for newly diagnosed high-grade glioma

A Phase I Study of Repeated Neural Stem Cell-Based Virotherapy and Standard Radiation and Chemotherapy for Newly Diagnosed High-Grade Glioma

Phase 1 Interventional Northwestern University · NCT06169280

This trial will test whether repeated doses of a neural stem cell–carried oncolytic virus (NSC-CRAd-S-pk7) are safe and feasible for adults newly diagnosed with high-grade glioma.

Quick facts

PhasePhase 1
Study typeInterventional
Enrollment20 (estimated)
Ages18 Years and up
SexAll
SponsorNorthwestern University Academic / other
Drugs / interventionschemotherapy, radiation
Locations1 site (Chicago, Illinois)
Trial IDNCT06169280 on ClinicalTrials.gov

What this trial studies

Participants undergo a diagnostic biopsy with an intraoperative injection of NSC-CRAd-S-pk7, then have tumor resection about two weeks later with a second intraoperative dose and placement of a catheter for subsequent dosing. Standard chemoradiotherapy (daily radiotherapy with concomitant temozolomide) begins after surgical recovery, with a third and fourth viral dose delivered through the implanted catheter timed during and at the end of radiotherapy; the formal DLT window begins with dose #3 because radiation activates the survivin promoter. Additional monthly doses are given through the catheter for a planned total treatment series, and patients are followed for safety, tumor response, time to progression, overall survival, and quality of life. The trial is a Phase 1, dose/feasibility-focused protocol conducted at a single center to characterize tolerability and preliminary signals of activity.

Who should consider this trial

Good fit: Adults (≥18 years) with newly diagnosed WHO grade 3 or 4 high-grade glioma whose tumor is accessible for injection, who plan to undergo standard chemoradiation, have WHO performance status < 2, and meet required laboratory and steroid criteria.

Not a fit: Patients with tumors in the brainstem or contained within the ventricular system, those unable to undergo biopsy/resection or standard chemoradiation, or those with poor performance status or significant organ dysfunction are unlikely to benefit from this protocol.

Why it matters

Potential benefit: If successful, this approach could improve local tumor control and potentially extend progression-free and overall survival by delivering oncolytic virus directly to tumor sites.

How similar studies have performed: Early-phase trials of oncolytic viruses and some cell-carrier strategies have reported preliminary safety and occasional tumor responses, but repeated NSC-CRAd-S-pk7 delivery in this schedule is largely novel and has limited prior clinical data.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Diagnosis of a high-grade glioma (WHO grade 3 or grade 4).
* Patients must have presumed high grade glioma (WHO grade 3 or 4) based on clinical and radiologic evaluation for registration.
* A pathologic confirmation of high grade glioma must be made at the time of stereotactic biopsy prior to NSC-CRAd-S-pk7 injection; if this is not possible, the injection will not be performed and the subject will no longer be eligible for the study).
* Tumor must be accessible for injection and must not be located in the brainstem or contained within the ventricular system.
* Planning to undergo standard radiation/chemotherapy.
* 18 years of age or older.
* Performance status (PS) must be WHO PS of \< 2.
* Stable or decreasing dose of corticosteroids equivalent to ≤ 6 mg/day for the 5 days prior to inclusion
* Serum glutamic-oxaloacetic transaminase (SGOT or AST) \< 3x upper limit of normal
* Serum creatinine \< 2mg/dl
* Platelets \> 100,000/mm3 and white blood cells (WBCs) \> 3000/mm3

Exclusion Criteria:

* Prior or ongoing liver disease including known cirrhosis.
* Known hepatitis B or C infection, known HIV infection.
* Chronic use of immunosuppressive drugs (with exception of corticosteroids required for mass effect).
* Acute viral, bacterial or fungal infections requiring therapy.
* Pregnant or breast-feeding patients.
* Evidence of metastatic disease or other malignancy (except squamous or basal cell skin cancers).
* Prior radiation therapy to the brain or prior treatment for brain tumor.

Where this trial is running

Chicago, Illinois

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Glioma, MalignantNew Diagnosis TumorVirotherapyNSC-CRAd-S-pk7
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.