Profiling blood and bone marrow in adults with newly diagnosed acute myeloid leukemia during chemotherapy

Single-cell Dynamic Profiling in Adults With Newly Diagnosed Acute Myeloid Leukemia Treated With Intensive Chemotherapy. A THEMA Study"

Observational Assistance Publique - Hôpitaux de Paris · NCT05304156

This study is trying to see how chemotherapy affects blood and bone marrow in adults with newly diagnosed acute myeloid leukemia to help improve treatment outcomes.

Quick facts

Study typeObservational
Enrollment200 (estimated)
Ages18 Years and up
SexAll
SponsorAssistance Publique - Hôpitaux de Paris Academic / other
Drugs / interventionsgemtuzumab, chemotherapy
Locations2 sites (Bobigny and 1 other locations)
Trial IDNCT05304156 on ClinicalTrials.gov

What this trial studies

This observational study aims to understand the molecular and cellular mechanisms of chemotherapy in adults with newly diagnosed acute myeloid leukemia (AML). By sequentially sampling peripheral blood and performing early bone marrow reassessments during the first course of intensive chemotherapy, researchers will utilize single-cell RNA sequencing and multiparameter flow cytometry. The goal is to correlate the dynamic changes in cancer cell phenotypes with clinical outcomes such as remission achievement and relapse-free survival. This approach may provide insights into optimizing chemotherapy treatments for AML patients.

Who should consider this trial

Good fit: Ideal candidates are adults aged 18 and older with newly diagnosed acute myeloid leukemia who are eligible for intensive chemotherapy.

Not a fit: Patients who have received prior treatment for AML or those with severe health issues preventing intensive chemotherapy may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to improved chemotherapy strategies and better outcomes for patients with acute myeloid leukemia.

How similar studies have performed: While the specific approach of this study is novel, similar studies have shown promise in understanding chemotherapy responses in other cancers.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* aged ≥18 years old,
* have a newly diagnosed AML according to WHO criteria

  o patients with AML related to prior chemotherapy or radiotherapy for another cancer will be eligible,
* have signed the informed consent form of the e-THEMA observatory trial
* have ≥10% blasts (blasts+myeloblasts) on the peripheral blood smear at screening,
* have ≥20% blasts on the bone marrow smear at screening,
* have not received any treatment for AML except for hydroxyurea and/or 6-mercaptopurine and steroids

  o Patients having previous treatments for antecedent myeloid neoplasms including hypomethylating agents remain eligible,
* Eligible to intensive chemotherapy, due to general health status,
* ECOG performance status ≤ 2,
* Patient is planned to receive anthracycline (daunorubicin \[DNR\] or idarubicine \[IDA\]) - cytarabine 7+3 with or without gemtuzumab ozogamycin (GO) or midostaurine, or CPX-351 as first induction course,
* Weighing 50 kg or more (compliance to Loi Jardé for PB sampling),
* Written informed consent obtained prior to any screening procedures,
* Eligible for National Health Insurance in France.

Exclusion Criteria:

* Suspected or proven acute promyelocytic leukemia based on morphology, karyotype or molecular assay,
* Failure to perform bone marrow aspiration at diagnosis,
* Unstable angina, New York Heart Association (NYHA) class 3 or 4 congestive heart failure,
* Prior anthracycline exposure more than 360 mg/m²,
* Previous therapy for AML with any other investigational agent or cytotoxic drug, within 28 days before starting treatment. Only hydroxyurea is permitted for the control of blood counts. Treatments for an antecedent myeloid neoplasm (MDS or MPN) are not considered as exclusion criteria.
* Women who are pregnant or breastfeeding.
* Any of concurrent severe and/or uncontrolled medical condition, which could compromise participation in the study.
* Enrolment in a clinical trial which could compromise participation in the study.

Where this trial is running

Bobigny and 1 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Acute Myeloid Leukemia
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.