Prebiotic powder to support sleep, mood, and thinking in 3–5-year-olds

The Impact of Chronic Consumption of Prebiotics on Cognitive and Affective Outcomes, Gut Microbiome Diversity and Composition in 3-5 Year Olds: a Double-blind Placebo-controlled, Parallel-groups Randomised Controlled Trial

NA · University of Reading · NCT07401212

We will test whether taking 5 g/day of a prebiotic powder for 8 weeks helps sleep, temperament, emotion regulation, and thinking skills and changes gut bacteria in healthy 3–5‑year‑old children who eat less fiber than recommended.

Quick facts

PhaseNA
Study typeInterventional
Enrollment106 (estimated)
Ages3 Years to 5 Years
SexAll
SponsorUniversity of Reading (other)
Locations1 site (Reading, Berkshire)
Trial IDNCT07401212 on ClinicalTrials.gov

What this trial studies

This is a double-blind, randomized, placebo-controlled parallel trial enrolling 106 children aged 3–5 years with lower-than-recommended dietary fiber intake. Participants are randomized to consume 5 g/day of a prebiotic powder or a matched placebo for 8 weeks, with cognitive and behavioral measures taken before and after supplementation. Stool samples are collected at baseline and week 8 to analyze microbiome diversity and composition. A 4-week washout is followed by online parent questionnaires at week 12 to capture any lasting changes.

Who should consider this trial

Good fit: Healthy children aged 3–5 years (36–71 months) whose daily fiber intake is below age-specific recommendations, without major chronic illnesses, and with no recent antibiotic or probiotic use are ideal candidates.

Not a fit: Children with significant chronic gastrointestinal, neurodevelopmental, immunological, psychiatric, or metabolic disorders, those with food allergies or intolerances, or those who recently used antibiotics or probiotic supplements are unlikely to benefit from this intervention as studied.

Why it matters

Potential benefit: If successful, this could provide a simple, low-risk dietary approach to improve sleep, behavior, and cognitive outcomes in young children with low fiber intake.

How similar studies have performed: Some animal and adult studies suggest prebiotics can alter the microbiome and modestly affect mood or cognition, but clinical evidence in preschool-aged children is limited and inconsistent.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Parents (18 years and over) of generally healthy children aged 3-5-years-old (36-71 months) with lower dietary fibre intake than the recommended daily amount (10.4g/day in 18-47-month-olds and less than 12.6g/day in 48-60-month-olds).

Exclusion Criteria:

* Children with a significant acute or chronic co-existing illness (including inflammatory bowel disease, functional gastrointestinal disorders, coeliac disease etc, neurodevelopmental, immunological, psychiatric, or metabolic disorders.
* Children who have taken any pre/probiotic supplements or antibiotic treatment within the 4 weeks prior to enrolment.
* Children with food allergies and intolerances

Where this trial is running

Reading, Berkshire

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.

View on ClinicalTrials.gov →

Conditions: Temperament, Sleep, Cognition, Emotion Regulation, prebiotics, emotion regulation, gut microbiome diversity, children

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.