Personalized antibiotic duration for high-risk febrile neutropenia with Gram-negative bloodstream infection

Appropriate Management of Bacteriemic Febrile Neutropenia in High-Risk Hematological Patients. Relationship Between Duration of Antibiotic Administration, Outcome and Resistance Profile

Not applicable Interventional Humanitas University · NCT07372131

This trial tests whether stopping antibiotics after a patient with a blood cancer and febrile neutropenia has been clinically stable for 72 hours is as safe and lets them spend more days off anti–Gram-negative antibiotics than the usual at least 10-day approach.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment172 (estimated)
Ages18 Years and up
SexAll
SponsorHumanitas University Academic / other
Drugs / interventionsCAR-T, chimeric antigen receptor, chemotherapy
Locations1 site (Rozzano, Milan)
Trial IDNCT07372131 on ClinicalTrials.gov

What this trial studies

This is a randomized, open-label trial that assigns hospitalized patients with hematologic malignancies and Gram-negative bacteraemia 1:1 to either stop antibiotics once they maintain clinical stability for 72 consecutive hours or to continue standard care with a suggested minimum of 10 days of therapy. Clinical stability is defined by sustained resolution of fever and stable or improving qSOFA score. A parallel microbiological sub-study uses shotgun metagenomic sequencing of stool, rectal swabs, and blood at baseline, end of treatment, and day 28 to compare microbiome recovery and acquisition of antimicrobial-resistance genes. All participants are followed clinically through day 28 to capture safety and antibiotic-free days outcomes.

Who should consider this trial

Good fit: Hospitalized adults with a hematologic malignancy receiving or planned for chemotherapy/BMT/CAR‑T who develop febrile neutropenia with documented Gram-negative bacteraemia and expected neutropenia ≥7 days are the intended candidates.

Not a fit: Patients with contextual pneumonia, other uncontrolled or complicated infections, non–Gram-negative bloodstream infections, or those who remain clinically unstable are unlikely to benefit from the personalized early-stop approach.

Why it matters

Potential benefit: If successful, this approach could reduce unnecessary antibiotic exposure, lower risk of resistant organisms, and speed recovery of the healthy gut microbiome for patients with febrile neutropenia and Gram-negative bloodstream infection.

How similar studies have performed: Shorter or stability-guided antibiotic courses have shown promise in some non-neutropenic infections, but randomized data specifically for high-risk febrile neutropenia with Gram-negative bacteraemia are limited, so this approach is relatively novel in this population.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Diagnosed with a hematologic malignancy that is candidate for treatment with chemotherapy or bone marrow transplantation or chimeric antigen receptor T cell therapy (CAR-T)
* Diagnosis of febrile neutropenia defined according to the guidelines of the Infectious Disease Society of America, IDSA; ref: Freifeld, A.G., et al., Clinical practice guideline for the use of antimicrobial agents in neutropenic patients with cancer: 2010 update by the infectious diseases society of america. Clin Infect Dis, 2011. 52(4): p. e56-93.) as: Fever: single record of oral temperature \>=38.3°C or a temperature \>=38.0°C sustained over a period of one hour; Neutropenia: absolute neutrophil count \< 1000 cells/microL; Expected duration of neutropenia \>= 7 days
* Diagnosis of bacteraemia defined by positive blood cultures (at least 1 vial positive for a non-contaminating microorganism)
* Isolation of Gram-Negative species

Exclusion Criteria:

* Contextual diagnosis of pneumonia
* Contextual diagnosis of intra-abdominal infection, in particular: neutropenic enterocolitis/typhlitis or biliary tract infection
* Persistently positive blood cultures at randomization
* Any condition that endangers the safety of the patient based on the judgment of the treating physician

Where this trial is running

Rozzano, Milan

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Bloodstream InfectionGram Negative InfectionsBacteraemia Caused by Gram-Negative BacteriaFebrile NeutropeniaAntibioticsHematological patients with febrile neutropeniaGram-negative
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.