Optimizing how to isolate extracellular vesicles from human follicular fluid
Optimization of the Isolation Protocol for Extracellular Vesicles (EVs) From Human Follicular Fluid
NA · University Hospital, Ghent · NCT06952751
This study is testing different ways to separate tiny particles from human follicular fluid to see which method works best for understanding their role in egg development.
Quick facts
| Phase | NA |
|---|---|
| Study type | Interventional |
| Enrollment | 4 (estimated) |
| Ages | 18 Years to 40 Years |
| Sex | Female |
| Sponsor | University Hospital, Ghent (other) |
| Locations | 1 site (Ghent) |
| Trial ID | NCT06952751 on ClinicalTrials.gov |
What this trial studies
This study aims to determine the most effective method for isolating extracellular vesicles (EVs) from human follicular fluid, which are believed to play a significant role in oocyte development. The research will compare three commonly used techniques: differential ultracentrifugation, OptiPrep™ density gradient ultracentrifugation, and size-exclusion chromatography, along with combined techniques. By identifying the optimal isolation protocol, the study seeks to enhance future research on the diagnostic and therapeutic potential of EVs and their contents. This foundational work is crucial for advancing our understanding of reproductive biology and potential clinical applications.
Who should consider this trial
Good fit: Ideal candidates for this study are patients scheduled for oocyte retrieval at Ghent University Hospital.
Not a fit: Patients with conditions such as endometriosis, adenomyosis, or polycystic ovary syndrome (PCOS) may not benefit from this study.
Why it matters
Potential benefit: If successful, this study could lead to improved methods for isolating EVs, enhancing research into their roles in fertility and potential therapeutic applications.
How similar studies have performed: While there are limited studies comparing EV isolation techniques in human follicular fluid, this approach is essential for establishing a foundation for future research.
Eligibility criteria
Show full inclusion / exclusion criteria
Inclusion Criteria: * Patients planned for an oocyte retrieval at Ghent University Hospital. Exclusion Criteria: * Diagnosis of endometriosis/adenomyosis * Diagnosis of PCOS (Polycystic Ovary Syndrome) * Macroscopic blood contamination at oocyte retrieval
Where this trial is running
Ghent
- Ghent University Hospital — Ghent, Belgium (RECRUITING)
Study contacts
- Study coordinator: Dominic Stoop, Prof. dr.
- Email: arg.studies@uzgent.be
- Phone: + 329 332 34 42
How to participate
- Review the eligibility criteria above with your treating physician.
- Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
- Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions: Extracellular Vesicles, Extracellular vesicles, Isolation