Methylation patterns and risk prediction for hepatitis B–related acute-on‑chronic liver failure

Analysis of Methylomic Features and Prognostic Risk Prediction in Patients With Hepatitis B-related Acute-on-chronic Liver Failure

Qilu Hospital of Shandong University · NCT07275554

This project will test whether methylation patterns can predict outcomes for people with hepatitis B–related acute-on‑chronic liver failure or cirrhosis.

Quick facts

Study typeObservational
Enrollment100 (estimated)
Ages18 Years and up
SexAll
SponsorQilu Hospital of Shandong University (other)
Locations1 site (Jinan, Shandong)
Trial IDNCT07275554 on ClinicalTrials.gov

What this trial studies

This observational effort will enroll patients with chronic hepatitis B who have HBV-related acute-on‑chronic liver failure or HBV-related cirrhosis at Qilu Hospital and collect clinical data and biosamples. Genome-wide DNA methylation (methylomics) profiling will be performed to identify methylation features linked to prognosis. The team will build and validate a prognostic risk-prediction classifier based on those methylation signatures. The aim is to develop a methylomics-based tool to support clinical assessment and improve diagnostic and prognostic efficiency for liver failure.

Who should consider this trial

Good fit: Adults with chronic hepatitis B who meet the 2018 Asia‑Pacific criteria for HBV-related acute-on‑chronic liver failure or who have HBV-related cirrhosis and can provide required clinical data at Qilu Hospital are ideal candidates.

Not a fit: Patients with co-infection by other hepatitis viruses, autoimmune liver disease, prior malignancy, inadequate clinical data, or non-HBV causes of liver failure are unlikely to benefit from this specific methylomics-based model.

Why it matters

Potential benefit: If successful, this could give clinicians a more accurate way to predict outcomes and tailor treatment for patients with HBV-related liver failure.

How similar studies have performed: Some prior work has identified methylation biomarkers linked to liver disease progression, but methylomics-based prognostic models specifically for HBV-related ACLF are novel and not yet widely validated.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Chronic hepatitis B was persistent hepatitis B surface antigen (HBsAg) seropositivity for more than six months.

Hepatitis B-related liver cirrhosis was diagnosed based on histological examination, typical features on ultrasound or radiological imaging, endoscopic findings of portal hypertension, and clinical history.

Hepatitis B-related acute-on-chronic liver failure meets the diagnostic criteria of the 2018 Asia-Pacific guidelines for the diagnosis and treatment of liver failure.Laboratory tests:Jaundice: Serum total bilirubin ≥ 85 µmol/L and coagulopathy \[International Normalized Ratio (INR) ≥ 1.5 or Prothrombin Activity (PTA) \< 40%\].

Exclusion Criteria:

* co-infection with other hepatitis viruses; history of hepatocellular carcinoma or other malignancies; concomitant autoimmune liver disease; or insufficient clinical data.

Where this trial is running

Jinan, Shandong

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.

View on ClinicalTrials.gov →

Conditions: HBV Related Acute-on-chronic Liver Failure, HBV-related Liver Cirrhosis, HBV

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.