Measuring Calprotectin Levels in Stool for Diagnosing Inflammatory Bowel Disease

ALPCO Calprotectin CLIA Assay - Measurement of Calprotectin Levels in Human Stool

Observational American Laboratory Products Company · NCT06810518

This study is testing a new stool test to see if it can help doctors diagnose Inflammatory Bowel Diseases like Crohn's and Ulcerative Colitis more accurately.

Quick facts

Study typeObservational
Enrollment220 (estimated)
Ages22 Years and up
SexAll
SponsorAmerican Laboratory Products Company Industry-sponsored
Drugs / interventionschemotherapy
Locations3 sites (Miami, Florida and 2 other locations)
Trial IDNCT06810518 on ClinicalTrials.gov

What this trial studies

This observational study aims to evaluate the ALPCO Calprotectin CLIA assay, which quantitatively measures calprotectin levels in human stool samples. Calprotectin is a protein biomarker that indicates mucosal inflammation, helping to diagnose Inflammatory Bowel Diseases (IBD) like Crohn's Disease and Ulcerative Colitis, and differentiate them from Irritable Bowel Syndrome (IBS). The study will compare the assay's sensitivity and specificity against gastroenterologist diagnoses, including colonoscopy results, to establish its predictive value. Participants will provide stool samples before or after diagnostic endoscopy to support the validation of the assay's cut-off values for clinical use.

Who should consider this trial

Good fit: Ideal candidates are adults aged 22 and older with suspected inflammatory bowel disease or irritable bowel syndrome presenting specific gastrointestinal symptoms.

Not a fit: Patients with known active intestinal cancer, active infections, or those currently receiving certain immunosuppressive therapies may not benefit from this study.

Why it matters

Potential benefit: If successful, this assay could provide a non-invasive method for accurately diagnosing IBD and distinguishing it from IBS, leading to better patient management.

How similar studies have performed: Previous studies have shown promising results with calprotectin as a biomarker for IBD, indicating that this approach is supported by existing research.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion criteria:

* Male or female, ≥22 years of age (adults)
* Suspected inflammatory bowel disease or irritable bowel syndrome with symptoms such as abdominal pain, diarrhea, altered appetite, weight loss, or anemia
* Able to provide a sample according to the sampling protocol within 72-24 hours prior to the scheduled diagnostic endoscopy or 3-30 days after the colonoscopy.
* Able to understand the study and the tasks required and sign the ICF

Exclusion criteria:

* Unable or unwilling to provide a stool specimen
* Known active intestinal cancer or in remission with abnormal levels (per physician assessment)
* Known active intestinal infection or history of treated intestinal infection with persistent abnormal levels (per physician assessment)
* IBD patients receiving chemotherapy or systemic immunosuppressive drugs without completing an 8-week washout period
* IBD patients who have previously diagnosed IBD managed with immunomodulators, 5-ASA or biologic therapies without completing an 8-week washout period for biologics and immunomodulators or 4 weeks for 5-ASA
* IBD patients who have previously been diagnosed with IBD and have undergone a surgical resection or diversion procedure.
* Known upper Gl disease such as esophagitis or gastritis that might influence the study test's ability to detect intestinal inflammatory disease
* Have taken NSAIDS (including aspirin) on 7 or more days during the 2 weeks before providing the sample
* Samples are not collected according to sampling protocol.
* Any condition that in the opinion of the investigator should preclude participation in the study.

Using an IRB approved consent form, all study participants will be advised of study requirements and potential risks associated with study participation.

Where this trial is running

Miami, Florida and 2 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Inflammatory Bowel Disease (Crohn&#39s Disease and Ulcerative Colitis)Inflammatory Bowel SyndromecalprotectinIBDIBS
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.