Investigating the microbiome and HPV status in couples facing fertility issues

Fertility and the Microbiome: an Observational Study and Randomized Placebo Controlled Double-blind Pilot Trial

Medical University of Graz · NCT05328999

This study is looking at how the bacteria in the body and HPV might affect couples trying to get pregnant, while also testing if probiotics can improve sperm quality in men with fertility issues.

Quick facts

Study typeObservational
Enrollment300 (estimated)
Ages18 Years to 50 Years
SexAll
SponsorMedical University of Graz (other)
Drugs / interventionsradiation
Locations1 site (Graz, Styria)
Trial IDNCT05328999 on ClinicalTrials.gov

What this trial studies

This observational study aims to explore the relationship between the microbiome and human papilloma virus (HPV) status in couples experiencing subfertility. The study will involve 150 couples over 24 months, collecting samples from various microbiomes and conducting HPV analysis. Additionally, a randomized placebo-controlled pilot study will assess the effects of probiotics on sperm quality in 30 subfertile men with asthenozoospermia. The outcomes will include time to pregnancy, pregnancy rates, and live birth rates.

Who should consider this trial

Good fit: Ideal candidates for this study are couples experiencing subfertility, whether due to primary or secondary infertility or recurrent pregnancy loss.

Not a fit: Patients who have recently taken probiotics, antibiotics, or nutritional supplements, or those with conditions affecting the investigation may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could provide insights into how microbiome health and HPV status affect fertility, potentially leading to new treatment options.

How similar studies have performed: While the association between microbiome and fertility is an emerging field, this specific approach combining microbiome analysis with HPV status in subfertile couples is relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Couples (Males and females) with subfertility, primary or secondary infertility, or recurrent pregnancy loss.

Exclusion Criteria:

* unable to consent
* diseases or conditions that might impact investigation
* intake of probiotics, antibiotics within last 3 months
* intake of nutritional supplements
* chemo therapy or radiation therapy
* excess alcohol intake
* smoking

Where this trial is running

Graz, Styria

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.

View on ClinicalTrials.gov →

Conditions: Subfertility, Asthenozoospermia

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.