Investigating emotional changes and genetics during puberty in girls

Negative Emotionality in Relation to Epigenetics of Estrogen Signaling During Puberty

Observational International Research Training Group 2804 · NCT06690866

This study is trying to see how hormonal changes during puberty affect emotions in girls by looking at their genes and stress levels.

Quick facts

Study typeObservational
Enrollment100 (estimated)
Ages8 Years to 17 Years
SexFemale
SponsorInternational Research Training Group 2804 Academic / other
Locations1 site (Tübingen, Baden-Wurttemberg)
Trial IDNCT06690866 on ClinicalTrials.gov

What this trial studies

This observational study examines the relationship between epigenetics and negative emotionality during puberty in healthy girls. It enrolls 50 pre-pubescent girls aged 8-10 and 50 post-pubescent girls aged 15-17, collecting data through questionnaires, neuroimaging (fMRI), and physiological stress assessments. Blood, saliva, and hair samples will be analyzed for hormone levels, DNA methylation, and gene expression related to stress and emotional responses. The goal is to understand how hormonal changes during puberty may influence mental health outcomes in females.

Who should consider this trial

Good fit: Ideal candidates are healthy girls aged 8-10 in early puberty or 15-17 in late puberty with normal body mass index.

Not a fit: Patients with neurological or psychiatric disorders, chronic medical conditions, or those undergoing hormonal treatments may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could provide insights into the biological factors contributing to increased mental health issues in females during puberty.

How similar studies have performed: While the specific interactions of epigenetics and emotionality during puberty are less explored, related studies have shown promising results in understanding hormonal influences on mental health.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* healthy girls
* aged between 8-10 and having pubertal stage 1 or between 15-17 and having pubertal stage 5
* normal body mass index according to age (between 5th and 85th percentile)
* non-smoking
* German language fluency
* Attending age-appropriate schools

Exclusion Criteria:

* neurological or psychiatric disease
* medical problems such as hormonal, metabolic, developmental or chronic diseases (e.g., congenital disorders, precocious puberty, polycystic ovarian syndrome, diabetes or congestive heart failure)
* any kind of hormonal, pharmacological or psychotropic treatment in the last three months
* engaging in competitive/extreme sports

Additional exclusion criteria for MRI:

* People with non-removable metal objects on or in the body
* Tattoos (if not MRI-incompatible according to expert guidelines)
* Pathological hearing or increased sensitivity to loud noises
* Claustrophobia
* Surgery less than three months ago
* Moderate or severe head injury

Where this trial is running

Tübingen, Baden-Wurttemberg

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions PubertyHealthyStressStress reactivityDNA methylationfMRI
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.