Intra-ovarian injection of stem cell-derived vesicles for premature ovarian failure

Safety and Feasibility Study of Intra-ovarian Injection of Bone Marrow Mesenchymal Stromal Cells-derived Extracellular Vesicles in Idiopathic Premature Ovarian Failure Patients: Clinical Trial Phase I

Phase1; Phase2 Interventional Royan Institute · NCT06202547

This study is testing if injecting special cell-made vesicles into the ovaries can help women with premature ovarian failure improve hormone levels and possibly restore their menstrual cycles.

Quick facts

PhasePhase1; Phase2
Study typeInterventional
Enrollment10 (estimated)
Ages20 Years to 38 Years
SexFemale
SponsorRoyan Institute Government
Locations1 site (Tehran)
Trial IDNCT06202547 on ClinicalTrials.gov

What this trial studies

This clinical trial investigates the effects of intra-ovarian injections of extracellular vesicles derived from mesenchymal stromal cells on women diagnosed with premature ovarian failure. The study aims to evaluate changes in hormone levels, specifically Follicle-Stimulating Hormone (FSH) and Anti-Mullerian Hormone (AMH), as well as the potential restoration of menstrual cycles over an 8-month follow-up period. A total of 10 eligible patients will receive the treatment, and their responses will be monitored for any side effects or complications. The trial is designed to provide insights into a novel therapeutic approach for this condition.

Who should consider this trial

Good fit: Ideal candidates for this study are women aged 20-38 with a diagnosis of premature ovarian failure and elevated FSH levels.

Not a fit: Patients with primary amenorrhea, congenital ovarian anomalies, or significant immunological and cardiovascular diseases may not benefit from this study.

Why it matters

Potential benefit: If successful, this treatment could restore ovarian function and menstrual cycles in women suffering from premature ovarian failure.

How similar studies have performed: While there have been studies on the use of stem cell-derived therapies, this specific approach using MSC-derived extracellular vesicles in premature ovarian failure is novel and has not been extensively tested.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Women between 20-38 years old
* Baseline serum level of follicle stimulating hormone (FSH) higher or equal to 25 IU/l at least twice with an interval of 3 or 4 months
* At least one year has passed since secondary amenorrhea and premature ovarian failure (POF) diagnosis
* Normal karyotype and fragile X messenger ribonucleoprotein 1 (FMR1) gene

Exclusion Criteria:

* Primary amenorrhea
* Congenital anomaly of the ovary
* Thyroid disease Immune system diseases such as lupus, etc.
* Previous and/or family history of ovarian tumor
* Previous and/or family history of suffering from major diseases in the past and present such as cancer
* Positive serological evidence regarding previous or current hepatitis B and C, Human T-lymphotropic virus 1, Human immunodeficiency virus (HIV), Syphilis Disturbance in the normal range of laboratory tests as levels of Hemoglobin Subunit Alpha 1 (HbA1), Alanine transaminase (ALT), The aspartate aminotransferase (AST), the number of white blood cells (WBCs), The creatinine (Cr), International normalised ratio (INR) , Platelets (Plt), Hematocrit (Hct) tests.
* History of specific systemic disease (rheumatology, endocrine, cardiovascular, etc.)
* Severe endometriosis (stage III and IV)
* Small and non-injectable ovaries Lack of patient satisfaction
* The patient's unwillingness to continue participating in the study

Where this trial is running

Tehran

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Premature Ovarian FailureIntra-ovarian injectionMesenchymal stromal cells-derived extracellular vesicles
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.