Indocyanine green for sentinel lymph node mapping in breast cancer

INFLUENCE II - a Pilot Study for Implementation Identification of Sentinel Lymph Nodes in Breast Cancer Patients Through Non-invasively FLUorEsceNt Imaging Using indoCyaninE Green: a Pilot Study for Implementation

Not applicable Interventional Casa di Cura Dott. Pederzoli · NCT07441733

This pilot tests whether indocyanine green (ICG) fluorescent imaging can be used for sentinel lymph node mapping in people with clinically node-negative breast cancer instead of or alongside technetium (99mTc).

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment40 (estimated)
Ages18 Years and up
SexFemale
SponsorCasa di Cura Dott. Pederzoli Academic / other
Locations1 site (Peschiera del Garda, Verona)
Trial IDNCT07441733 on ClinicalTrials.gov

What this trial studies

This single-institution pilot uses a pre- and post-implementation design to compare the standard 99mTc-nanocolloid sentinel node procedure with ICG fluorescence guidance. During a transition period participating surgeons receive on-site training, proctoring, and ongoing support from experienced ICG users before full implementation. After implementation, 5 mg (2 ml) ICG will be injected periareolarly under general anesthesia and sentinel nodes visualized with a fluorescence camera; outcomes including SLN identification rate, number of nodes removed, detection time, operative time, complications, and one-year loco-regional recurrence will be recorded. The study also collects surgeon expectations and post-implementation experiences to identify barriers and success factors for adopting ICG.

Who should consider this trial

Good fit: People with biopsy-confirmed DCIS or invasive T1–T3 breast cancer who are clinically node-negative on preoperative axillary ultrasound and scheduled for breast surgery with sentinel lymph node biopsy via an axillary incision who can give informed consent are ideal candidates.

Not a fit: Patients requiring SLNB via a mastectomy incision, those with prior axillary lymph node dissection, known allergy to ICG/iodine, pregnant or breastfeeding individuals, or those unable to comply with the protocol are unlikely to benefit from participation.

Why it matters

Potential benefit: If successful, ICG could simplify and speed up sentinel node mapping and reduce reliance on radioactive tracers, benefiting patients and surgical workflow.

How similar studies have performed: Multiple single-center and multicenter reports have shown that ICG fluorescence often achieves SLN detection rates comparable to technetium and can be a viable non-radioactive alternative.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Clinically node-negative, DCIS or invasive stage T1-T3 breast cancer confirmed by biopsy
* Preoperative axillary ultrasound to confirm clinical node-negative status
* Indication for breast cancer surgery with SLNB via axillar incision
* Written informed consent according to national regulations

Exclusion Criteria:

* Patients \< 18 years old.
* Indication for breast cancer surgery with SLNB via mastectomy incision
* Known allergy for ICG, intravenous contrast or iodine
* History of axillary lymph node dissection
* Pregnancy or breast-feeding
* Psychological, familial, sociological or geographical factors that could potentially hamper compliance with the study protocol

Where this trial is running

Peschiera del Garda, Verona

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Breast Cancerbreast cancerlymph node99mTcICG
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.