Imaging brain fluids during breathing tasks

Neuroimaging the Impact of Respiration and Respiratory-gated Neuromodulation on Human Glymphatic Physiology

NA · Boston University Charles River Campus · NCT05180981

This study is testing how different breathing patterns and a nerve stimulation technique affect the flow of fluid around the brain in people to learn more about brain health.

Quick facts

PhaseNA
Study typeInterventional
Enrollment80 (estimated)
Ages18 Years to 70 Years
SexAll
SponsorBoston University Charles River Campus (other)
Locations2 sites (Boston, Massachusetts and 1 other locations)
Trial IDNCT05180981 on ClinicalTrials.gov

What this trial studies

This study investigates how breathing patterns affect the flow of cerebrospinal fluid (CSF) around the brain using high-resolution magnetic resonance imaging (MRI). Participants will engage in specific breathing tasks or receive transcutaneous vagal nerve stimulation while undergoing MRI scans. The goal is to understand the relationship between breathing and CSF dynamics, which are crucial for brain health. The study will involve two groups: one focusing on paced breathing and the other on vagal nerve stimulation, with imaging conducted at Massachusetts General Hospital.

Who should consider this trial

Good fit: Ideal candidates are healthy adults aged 18-70 without neurological or psychiatric disorders.

Not a fit: Patients with major head trauma, neurological or psychiatric disorders, or those with implanted electronic devices may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could enhance our understanding of brain health and the role of breathing in cerebrospinal fluid dynamics.

How similar studies have performed: While the specific approach of imaging CSF flow during breathing is novel, related studies on CSF dynamics have shown promising results.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Adult age 18-70 years

Exclusion Criteria:

* No ferrous metal implanted in head or body
* No history of major head trauma
* No neurological or psychiatric disorder
* Not using medication that affects brain function
* No implanted electronic devices (e.g. pacemaker)
* No implant that poses an MR contraindication
* Not pregnant, suspect they are pregnant, or seeking to become pregnant
* Not claustrophobic
* No piercings or jewelry that cannot be removed
* Does not weigh more than 250 pounds
* Normal or contact-corrected normal vision

Exclusion for transcutaneous vagus nerve stimulation (tVNS) arm:

* Conditions of skin or anatomy that affect left auricle or forehead skin and could impact placement of electrodes for tVNS or forehead stimulation
* Diagnosis of significant cardiovascular or cerebrovascular disease \[e.g. congestive heart failure, stroke, cardiac conduction disorders (including: bundle branch block, heart block, long Q-T syndrome), history of asystole or non-sustained ventricular tachycardia.
* Bradycardia (defined as resting heart rate \<50 bpm)
* Hypotension defined as blood pressure \<90/60 mmHg

Where this trial is running

Boston, Massachusetts and 1 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.

View on ClinicalTrials.gov →

Conditions: Healthy, Glymphatics, Cerebrospinal fluid

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.