How short dehydration and rehydration affect body function in middle-aged adults
Official title: Impact of Mild Dehydration and Subsequent Rehydration on Cellular Stress and Physiological Performance
Run by Texas Tech University · NCT07084675 · Data updated 2026-11-12
This will test whether short periods of mild dehydration followed by rehydration change inflammation markers and physical measures like sleep, fasting glucose, and strength in adults 45–65.
Quick facts
| Study type | Interventional |
|---|---|
| Ages | 45 Years to 65 Years |
| Sex | All |
| Enrollment | 15 (estimated) |
| Sponsor | Texas Tech University Academic / other |
| Locations | 1 site (Lubbock, Texas) |
| Status | Recruiting |
| Started | July 15, 2025 |
| Main results expected | December 2025 |
| Study ends | December 2026 |
| Registry record updated | September 17, 2025 |
| Trial ID | NCT07084675 on ClinicalTrials.gov |
Is this trial a fit for you?
✓Likely a good fit
Ideal candidates are apparently healthy adults aged 45–65 with BMI under 30 and body weight at least 110 pounds who are not pregnant and do not have diabetes, kidney disease, or hypertension.
✕Probably not a fit
People with diabetes, chronic kidney disease, hypertension, those taking excluded medications that alter fluid balance, pregnant individuals, or those with BMI ≥30 are unlikely to benefit or be eligible.
A plain-English reading of the study's rules. Only the study team can confirm whether you qualify.
What this trial studies
This protocol tests the effects of a controlled 12-day hydration sequence—4 days of increased hydration, 4 days of mild dehydration (≈2–3% body-mass loss), and 4 days of rehydration—on molecular and functional outcomes in adults aged 45–65. Participants attend five lab visits for consent/familiarization, pre-hydration baseline, pre-dehydration testing, post-dehydration testing, and post-rehydration testing. Outcomes include H2O2, NF-κB signaling, mitochondrial fragmentation, and autophagy in circulating PBMCs, along with fasting glucose, sleep performance, and strength measures. The protocol enrolls apparently healthy adults (BMI <30, weight ≥110 lbs) and excludes pregnancy, diabetes, chronic kidney disease or kidney stones, hypertension, and use of medications that affect fluid balance; all visits occur at Texas Tech University in Lubbock, Texas.
Why it matters
Potential benefit: If successful, the results could clarify whether modest short-term hydration changes influence inflammation and physical function and help shape hydration recommendations for middle-aged adults.
How similar studies have gone: There is prior evidence linking hydration to physical performance and some metabolic markers, but using controlled short-term dehydration/rehydration with mechanistic PBMC measures is relatively novel and limited in prior work.
Who can join: the full rules
Show full inclusion / exclusion criteria
Inclusion Criteria: * Apparently Healthy * BMI \< 30 kg/m2 * Body weight is at least 110 lbs Exclusion Criteria: * Currently pregnant * Have Type 1 Diabetes or Type 2 Diabetes * Have chronic kidney disease or a history of kidney stones * Have hypertension * Take medications that can cause fluid retention, such as corticosteroids, chronic use of Nonsteroidal Anti-inflammatory Drugs (NSAIDs), Hormonal Therapies (e.g., estrogen, androgens or anabolic steroids), Antihypertensives, Psychiatric Medications (e.g., Lithium), Vasodilators * Take medications that reduce fluid retention: diuretics, and RAAS Inhibitors (e.g., ACE inhibitors and ARBs)
Where it's running
-
Texas Tech University
Lubbock, Texas, United States Recruiting
Study contacts
- Study coordinator: Hui Ying Luk, Ph.D.
- Email: huiying.luk@ttu.edu
- Phone: 806-834-0827
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How to take part
- Review the eligibility criteria above with your treating physician.
- Check the official listing on ClinicalTrials.gov for the latest contact details and recruitment status.
- Contact the study coordinator or principal investigator to ask for pre-screening. Pre-screening is free and never obligates you to enroll.