How one day of very low activity changes next-day exercise response

A Single Day of Inactivity on the Systemic Metabolic and Inflammatory Exercise Response

Not applicable Interventional Concordia University Wisconsin · NCT07429370

This project will test whether one day of very low activity changes how healthy 18–30-year-olds' bodies respond to exercise the next day.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment6 (estimated)
Ages18 Years to 30 Years
SexAll
SponsorConcordia University Wisconsin Academic / other
Locations1 site (Mequon, Wisconsin)
Trial IDNCT07429370 on ClinicalTrials.gov

What this trial studies

Healthy young adults will reduce their daily steps (to under 5,000) for one day and then complete a standardized bout of aerobic exercise while researchers collect blood and other samples. The study will measure circulating metabolomics and proteomics and compare RNA profiles at rest and during exercise after normal and low-activity days. Analyses will focus on carbohydrate utilization, inflammation-related pathways, metabolism, and innate immunity to explain altered exercise responses. The goal is to link a short period of inactivity to specific molecular changes that modify the physiological effects of exercise.

Who should consider this trial

Good fit: Ideal participants are healthy 18–30-year-olds without acute or chronic cardiopulmonary, liver, kidney, or metabolic disease, not taking metabolism-altering medications, non-smokers, and without orthopedic limitations.

Not a fit: People older than 30, those with chronic diseases, current smokers, or anyone taking metabolism-related medications (e.g., statins, metformin, GLP-1 agonists) are unlikely to match the study population and may not benefit from these findings.

Why it matters

Potential benefit: If successful, this work could identify biological signals to help tailor activity recommendations so people get more benefit from each exercise session.

How similar studies have performed: Earlier smaller studies have shown increased carbohydrate use and RNA changes after a day of reduced activity, but comprehensive metabolomics and proteomics profiling in this exact context is relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* • between the ages of 18-30 years old

Exclusion Criteria:

* • free from acute or chronic illness (cardiac, pulmonary, liver, or kidney abnormalities, cancer, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other known metabolic disorders)

  * do not take medications related to metabolism (i.e., statins, metformin, GLP-1 agonists)
  * free from orthopedic limitations (including any artificial joints)
  * currently smoke or participate in other forms of tobacco use.

Where this trial is running

Mequon, Wisconsin

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Inactivity, PhysicalExerciseMetabolism
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.