Gut microbiome after colorectal intestinal anastomosis

Analysis of the Composition of the Intestinal Microbiota and Its Evolution in Patients Post-intestinal Anastomosis

Observational Instituto de Investigación Sanitaria de la Fundación Jiménez Díaz · NCT07416760

This study will see if differences in the gut microbiome are linked to anastomotic leakage in adults undergoing colorectal intestinal anastomosis.

Quick facts

Study typeObservational
Enrollment30 (estimated)
Ages18 Years and up
SexAll
SponsorInstituto de Investigación Sanitaria de la Fundación Jiménez Díaz Academic / other
Locations1 site (Madrid)
Trial IDNCT07416760 on ClinicalTrials.gov

What this trial studies

This is a prospective, descriptive, non-interventional study enrolling adults who undergo colorectal intestinal anastomosis at a single hospital. Stool samples will be collected before surgical preparation, during the operation, and in the postoperative period to profile bacterial composition and diversity. Microbiome sequencing and bioinformatic analyses will compare patients who develop anastomotic dehiscence with those who do not. The study aims to correlate microbial patterns, including Lachnospiraceae abundance and overall diversity, with anastomotic outcomes.

Who should consider this trial

Good fit: Adults (≥18 years) in good functional status scheduled for elective colorectal intestinal anastomosis who can give informed consent and attend postoperative follow-up are ideal candidates.

Not a fit: Patients having emergency surgery, with intra-abdominal sepsis or prior dehiscence, severe immunosuppression, recent antibiotic use within 3 months, or who are pregnant or breastfeeding are unlikely to benefit from this observational analysis.

Why it matters

Potential benefit: If successful, the study could identify microbiome markers that predict higher risk of anastomotic leakage and help guide prevention or monitoring strategies.

How similar studies have performed: Previous observational studies have reported links between lower microbial diversity, higher Lachnospiraceae abundance, and anastomotic leakage, but findings are correlational and causality remains unproven.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Patients who have undergone colorectal intestinal anastomosis.
* Signing of the informed consent form.
* Age ≥18 years with good functional status.
* Availability for postoperative follow-up.

Exclusion Criteria:

* Emergency surgery.
* Intra-abdominal sepsis or previous dehiscence.
* Severe immunosuppression.
* Use of antibiotics in the 3 months prior to preoperative preparation.
* Pregnancy or breastfeeding.

Where this trial is running

Madrid

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Abdominal SurgeryMicrobiomeintestinal anastomosisdehiscence
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.