Free vitamin D and steroid metabolism in endocrine and kidney conditions

Free Fraction Vitamin D Measurements to Improve Understanding of Steroid Metabolism in Endocrine Disorders

Observational Universitaire Ziekenhuizen KU Leuven · NCT07268742

This project will measure free (unbound) vitamin D and related steroid hormones in adults — including healthy people, pregnant women, and patients with endocrine or kidney disorders — to see how processing differs between groups.

Quick facts

Study typeObservational
Enrollment930 (estimated)
Ages18 Years and up
SexAll
SponsorUniversitaire Ziekenhuizen KU Leuven Academic / other
Locations1 site (Leuven)
Trial IDNCT07268742 on ClinicalTrials.gov

What this trial studies

This observational study collects blood samples from adults across several cohorts (healthy controls, people with obesity, chronic kidney disease stage ≥3a, first-trimester pregnancy, primary hyperparathyroidism scheduled for surgery, and complex calcium/phosphate disorders) to measure free and total vitamin D and related steroid markers. Laboratory assays will quantify free vitamin D, total vitamin D, vitamin D binding protein, and selected steroid metabolites to characterize binding and metabolic patterns. The study compares these measures between groups to determine whether total vitamin D testing reflects biologically available free vitamin D in different clinical contexts. Findings are intended to clarify how vitamin D results should be interpreted in pregnancy and in various endocrine and renal disorders.

Who should consider this trial

Good fit: Adults aged 18 or older who can consent and fit one of the study cohorts — healthy controls, people with BMI ≥25 kg/m² (obesity cohort), chronic kidney disease stage ≥3a, women in first-trimester pregnancy, patients with primary hyperparathyroidism scheduled for parathyroidectomy, or those with complex calcium/phosphate disorders — are ideal candidates.

Not a fit: People under 18, those unable to give informed consent, or individuals whose clinical profile does not match any cohort (for example those taking excluded vitamin D or calcium supplementation when applicable) are unlikely to benefit from participation.

Why it matters

Potential benefit: If successful, this could help doctors interpret vitamin D tests more accurately and lead to better diagnosis and treatment for people with pregnancy, endocrine, or kidney conditions.

How similar studies have performed: Previous research on the free vitamin D or 'free hormone' hypothesis has produced promising but mixed results, so the approach is emerging but not yet an established clinical standard.

Eligibility criteria

Show full inclusion / exclusion criteria
1. General inclusion Criteria:

   \- 18 years or above
2. General exclusion Criteria:

   * Below 18 years of age
   * Individuals incapable of providing informed consent
3. Cohort inclusion criteria

   * Obesity: BMI ≥ 25 kg/m2
   * Chronic Kidney Disease: ≥ CKD stage 3a (eGFR \< 60 mL/min/1.73m2)
   * Pregnancy: in trimester 1 of pregnancy
   * Primary hyperparathyroidism: diagnosis at UZ Leuven and scheduled for parathyroidectomy at UZ Leuven
   * Complex calcium and phosphate disorders: diagnosis at UZ Leuven
4. Cohort exclusion criteria

   * Healthy controls: BMI below 18 kg/m² or above 25 kg/m², pregnancy, chronic kidney disease (independent of staging), primary hyperparathyroidism and vitamin D and/or calcium supplementation
   * Obesity: chronic kidney disease (independent of staging), pregnancy, primary hyperparathyroidism
   * Chronic Kidney Disease: BMI below 18 kg/m² or above 30 kg/m², pregnancy, primary hyperparathyroidism
   * Pregnancy: BMI below 18 kg/m² or above 30 kg/m², chronic kidney disease (independent of staging), primary hyperparathyroidism
   * Primary hyperparathyroidism: BMI below 18 kg/m² or above 30 kg/m², pregnancy, chronic kidney disease (independent of staging), secondary hyperparathyroidism

Where this trial is running

Leuven

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Vitamin DVitamin D and Calcium HomeostasisFree vitamin DFree hormone hypothesis
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.