Examining how sex differences affect heart fat and function

Mechanism and Modulation of Sex Differences in Myocardial Steatosis Induced Left Ventricular Dysfunction

Phase 4 Interventional The University of Texas at Arlington · NCT04671966

This study is testing how heart fat and function differ between healthy men and premenopausal women to better understand how sex affects heart health.

Quick facts

PhasePhase 4
Study typeInterventional
Enrollment100 (estimated)
Ages18 Years to 30 Years
SexAll
SponsorThe University of Texas at Arlington Academic / other
Drugs / interventionsmethotrexate
Locations1 site (Arlington, Texas)
Trial IDNCT04671966 on ClinicalTrials.gov

What this trial studies

This study investigates the impact of myocardial steatosis on left ventricular dysfunction, focusing on sex differences between healthy men and premenopausal women. Participants will undergo cardiac MRI and spectroscopy to assess heart structure and fat metabolism after a two-day fasting period to increase myocardial triglyceride content. The study will also evaluate metabolic health and hormonal levels through blood samples. The goal is to better understand how biological sex influences heart health and fat deposition.

Who should consider this trial

Good fit: Ideal candidates are healthy men and premenopausal women aged 18-50 with specific blood pressure and BMI criteria.

Not a fit: Patients with a history of estrogen-dependent neoplasms, cardiovascular disease, or those on hormone therapy may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to improved understanding and treatment strategies for heart diseases that consider sex differences.

How similar studies have performed: While there is ongoing research into sex differences in cardiovascular health, this specific approach focusing on myocardial steatosis and fasting is relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* blood pressure \<140/90 mmHg
* BMI between 18.5 and 30 kg/m2
* sedentary or recreationally active (\<3 days of vigorous aerobic exercise each week)
* no use of oral contraceptives hormone therapy, or other medications that might influence cardiovascular function
* nonsmokers.

Exclusion Criteria:

* Women will be excluded with

  1. history of or active estrogen-dependent neoplasms, acute liver or gallbladder disease, vaginal bleeding, venous thromboembolism, hypertriglyceridemia, and CVD
  2. known allergy to transdermal patch, GnRHant (i.e., hypersensitivity to cetrorelix, extrinsic peptide hormones, mannitol, GnRH, benzyl alcohol - the vehicle for injection of cetrorelix)
  3. history of stomach ulcer or bleeding
  4. other contraindications to hormone replacement therapy or GnRHant, (i.e., taking Levodopa, medications that can inhibit folate metabolism including methotrexate).

Other conditions for which individuals will be excluded from the study include:

diabetes active infection history of seizures or disease that affects the nervous system sepsis an abnormal resting ECG contraindications to MRI pregnant or planning to become pregnant smoking history illicit drug use (excluding occasional marijuana).

Where this trial is running

Arlington, Texas

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Heart DiseasesLeft Ventricular Dysfunctionmyocardial steatosisfastingestrogenbiological sex as a variable
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.