Evaluating tests for detecting drug-resistant gonorrhea

Master Protocol for Evaluating Multiple Infection Diagnostics for Ciprofloxacin-Resistant Neisseria Gonorrhoeae

Observational Duke University · NCT06815536

This study is testing new ways to see if certain tests can accurately find a gene that makes gonorrhea resistant to a common antibiotic in samples from patients.

Quick facts

Study typeObservational
Enrollment3291 (estimated)
SexAll
SponsorDuke University Academic / other
Locations8 sites (San Francisco, California and 7 other locations)
Trial IDNCT06815536 on ClinicalTrials.gov

What this trial studies

This observational study aims to assess the accuracy of several investigational tests in identifying a specific gene mutation (gyrA 91F) that indicates ciprofloxacin resistance in Neisseria gonorrhoeae. The study will utilize clinical specimens collected during routine care that test positive for N. gonorrhoeae using FDA-cleared molecular assays. A total of 311 urine, 496 vaginal swab, and 469 pharyngeal specimens will be analyzed using both the investigational tests and a reference standard of Sanger sequencing. The goal is to determine if these tests can reliably detect the mutation compared to the sequencing results.

Who should consider this trial

Good fit: Ideal candidates for this study are individuals who test positive for Neisseria gonorrhoeae and have sufficient specimen volume for testing.

Not a fit: Patients whose specimens are not compatible with the investigational tests or lack proper labeling for epidemiologic data may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to improved diagnostic methods for identifying drug-resistant gonorrhea, allowing for more effective treatment options.

How similar studies have performed: Other studies have shown promise in using genetic testing for identifying drug resistance in bacterial infections, suggesting potential success for this approach.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Be N. gonorrhoeae-positive on an FDA-cleared molecular assay
* Have sufficient N. gonorrhoeae-positive specimen volume for testing using the corresponding investigational reflex test and genetic sequencing
* Undergo proper handling and storage conditions

Exclusion Criteria:

* The N. gonorrhoeae-positive specimen media is not compatible with the investigational reflex test(s) at the laboratory site
* The N. gonorrhoeae-positive specimen is not clearly labeled by the laboratory to link to basic epidemiologic data (age, sex) and source

Where this trial is running

San Francisco, California and 7 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Neisseria Gonorrheae Infectionmutant allele gyrA 91Fwildtype allele gyrA 91Sciprofloxacin-resistant Neisseria gonorrhoeae
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.