Evaluating 4-aminopyridine for nerve injury diagnosis

Pharmaco-Diagnostic Crossover Trial for Peripheral Nerve Continuity After Trauma

Phase 2 Interventional University of Arizona · NCT06003166

This study is testing if a single dose of 4-aminopyridine can help doctors better diagnose the severity of nerve injuries in people.

Quick facts

PhasePhase 2
Study typeInterventional
Enrollment68 (estimated)
Ages18 Years to 90 Years
SexAll
SponsorUniversity of Arizona Academic / other
Locations1 site (Tucson, Arizona)
Trial IDNCT06003166 on ClinicalTrials.gov

What this trial studies

This clinical trial investigates the effectiveness of a single dose of 4-aminopyridine (4-AP) in diagnosing the severity of peripheral nerve injuries, specifically distinguishing between severing and non-severing injuries. Participants will be randomized into two groups to receive either the drug or a placebo, followed by a crossover to the alternate treatment. The study will involve comprehensive sensory and motor evaluations, electrodiagnostic testing, and serum level assessments of 4-AP over a three-hour period. The goal is to determine if 4-AP can expedite the identification of incomplete nerve injuries compared to standard diagnostic methods.

Who should consider this trial

Good fit: Ideal candidates include adults aged 18-90 with unclear continuity of peripheral nerves due to trauma affecting two or fewer limbs.

Not a fit: Patients with distracting injuries or those scheduled for surgical exploration of the nerve within 48 hours may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to faster and more accurate diagnoses of peripheral nerve injuries, improving patient outcomes.

How similar studies have performed: While the use of 4-AP in this context is novel, similar studies have shown promise in enhancing nerve injury assessments.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Patients with trauma involving two or less limbs where the continuity of a given peripheral nerve or nerves is unclear on presenting physical examination.
* Closed soft tissue envelope obscuring direct observation of the continuity of the affected nerve.
* Cognitive ability to report sensory and motor deficit during examination.
* Eligible for standard of care plan of monitoring vs surgical exploration of the nerve.
* Adults subject aged 18-90
* Known limb trauma which resulted in nerve injury (aim 1) or post-operative/post intervention nerve injury (aim 2).
* Ability to give written informed consent.
* Availability for all testing days and main trial day.

Exclusion Criteria:

* Distracting injury which prevents adequate examination.
* Plan for surgical exploration of the nerve during the ensuing 48 hours.
* Plan for surgical exploration of the nerve as part of another surgical procedure within 48 hours of evaluation.
* Intoxication during examination or evidence of cognitive deficit that emerges during examination.
* History of multiple sclerosis, stroke or any other diagnosed neurological disorder
* History of hypersensitivity to AMPYRA® or 4-aminopyridine
* Current use of aminopyridine medications, including other compounded 4-AP
* Renal impairment based on calculated GFR (GFR\<80 mL/min). This laboratory value is measured in all inpatient trauma patients as part of the standard of care.
* History of difficult compliance with timely follow up or plan to seek care at another institution closer to home.
* Patients outside the age range or unable to consent.
* Patients with a known history of a seizure disorder (4AP overdose can, in selected cases, result in limited seizure activity).
* Patients with a concomitant traumatic brain injury.
* Patients unable to communicate return or loss of sensation.
* Patients unable to exhibit motor control on the affected limb at baseline.
* Patients unwilling to complete the study requirements.
* Patients with injuries too extensive to isolate a single nerve(s) for testing.
* Patients currently taking organic cat-ion transporter 2 (OCT2) inhibitors, e.g. Cimetidine.
* Pregnancy, breastfeeding or incarcerated individuals.

Where this trial is running

Tucson, Arizona

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Peripheral Nerve InjuryCrush Injury4 aminopyridineperipheral nervecrush injury
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.