Effects of Ocrelizumab on Immune Cells in Multiple Sclerosis

Identifying Ocrelizumab-resistant Lymphocytes in Lymphoid Tissue in Multiple Sclerosis

This study is testing how the medication ocrelizumab affects certain immune cells in people with relapsing-remitting multiple sclerosis to see how it might help them in the long run.

Quick facts

• Phase: Phase 4
• Study type: Interventional
• Enrollment: 5 (estimated)
• Ages: 18 Years to 65 Years
• Sex: All
• Sponsor: University of California, San Francisco (Academic / other)
• Drugs / interventions: ocrelizumab, methotrexate, cyclophosphamide
• Locations: 1 site (San Francisco, California)
• Trial ID: NCT06495593 on ClinicalTrials.gov

What this trial studies

This phase IV observational study investigates how the B cell-depleting therapy ocrelizumab affects B cells and T cells in patients with relapsing-remitting multiple sclerosis (MS). Eligible participants, aged 18-65 and treatment-naïve, will undergo blood and lymph node fine needle aspiration before starting ocrelizumab and again three months post-treatment. The study aims to understand the extent of B cell elimination in lymph nodes, which are crucial for B cell activation, and its implications for long-term MS outcomes.

Who should consider this trial

Good fit: Ideal candidates are adults aged 18-65 with a diagnosis of relapsing-remitting MS who have not previously received disease-modifying therapy.

Not a fit: Patients with secondary progressive MS, primary progressive MS, or those who have previously been treated with disease-modifying therapies may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could enhance understanding of B cell dynamics in MS and improve treatment strategies for patients.

How similar studies have performed: Other studies have shown success with B cell-depleting therapies in MS, indicating a promising approach.

Eligibility criteria

Inclusion Criteria:

* Ability to provide written informed consent and be compliant with the study protocol
* The treating neurologist's independent medical assessment and decision to initiate the patient on ocrelizumab treatment as most appropriate standard of care for the patient
* Diagnosis of RR-MS with Expanded Disability Status Scale (EDSS) 0-5.5 at enrollment
* Treatment-naïve (i.e. no prior disease modifying therapy)
* Disease duration from the onset of MS symptoms: less than 15 years in patients with an EDSS greater than 5.0 at screening
* For women of childbearing potential: agreement to remain abstinent (refrain from heterosexual intercourse) or use acceptable contraceptive methods during the treatment period and for 6 months after the final dose of ocrelizumab

Exclusion Criteria:

MS Related:

* Diagnosis of secondary progressive MS without relapses for at least 1 year.
* Diagnosis of primary progressive MS.
* Prior treatment with any disease modifying therapy for MS.
* Previous or concurrent treatment with any investigational agent or treatment with any experimental procedure for MS (e.g., treatment for chronic cerebrospinal venous insufficiency).

Infection Related:

* Known presence of recurrent or chronic infection (e.g., HIV, syphilis, tuberculosis).
* History of recurrent aspiration pneumonia requiring antibiotic therapy.
* History or known presence of infectious causes of myelopathy (e.g., syphilis, Lyme disease, HTLV-1, herpes zoster myelopathy).
* Known active bacterial, viral, fungal, mycobacterial infection, or other infection (including tuberculosis or atypical mycobacterial disease, but excluding fungal infection of nail beds) or any major episode of infection requiring hospitalization or treatment with IV antibiotics within 4 weeks prior to baseline visit or oral antibiotics within 2 weeks prior to baseline visit.

Cancer Related:

- History of cancer, including solid tumors and hematological malignancies (except basal cell, in situ squamous cell carcinomas of the skin, and in situ carcinoma of the cervix or the uterus that have been excised and resolved with documented clean margins on pathology).

Women's health-related:

* Pregnant or lactating, or intending to become pregnant during the study
* Women of childbearing potential must have a negative serum or urine pregnancy test result within 14 days prior to initiation of study drug.

Other Medical Conditions:

* On systemic anti-coagulation or known blood clotting disorder.
* History of or currently active primary or secondary immunodeficiency.
* History of severe allergic or anaphylactic reactions to humanized or murine monoclonal antibodies.
* History of alcohol or other drug abuse within 24 weeks prior to enrollment.
* History or known presence of systemic autoimmune disorders, potentially causing progressive neurologic disease (e.g., lupus, anti-phospholipid antibody syndrome, Sjögren's syndrome, Behçet's disease).
* Any concomitant disease that may require chronic treatment with systemic corticosteroids or immunosuppressants during the course of the study.
* Significant, uncontrolled disease, as defined by AMA guidelines or similar, such as cardiovascular (including congestive heart failure - NYHA grade 3 or 4, cardiac arrhythmia), uncontrolled hypertension, pulmonary (including chronic obstructive pulmonary disease), renal, hepatic, endocrine (including uncontrolled diabetes mellitus), gastrointestinal, or any other significant disease.
* Known presence or history of other neurologic disorders
* Systemic corticosteroid therapy within 4 weeks prior to baseline.
* Contraindications for, or intolerance to, oral or IV corticosteroids, including IV methylprednisolone, according to the country label, including hypersensitivity to any of the treatment drug constituents.
* Previous treatment with cyclophosphamide, mitoxantrone, azathioprine, mycophenolate mofetil, cyclosporine, methotrexate, total body irradiation, or bone marrow transplantation.

Laboratory:

* Positive serum or urine β-hCG.
* Positive for hepatitis B (hepatitis B surface antigen \[HBsAg\] positive or hepatitis B core antibody \[total HBcAb\] confirmed by positive viral DNA polymerase chain reaction \[PCR\]).
* AST or ALT more than 2 times the upper limit of normal.
* Platelet count below lower limit of normal.
* Total white blood cell count, including differential counts, below lower limit of normal.
* Absolute neutrophil count below lower limit of normal.
* Lymphocyte count below lower level of normal.
* Levels of serum IgG 18% below the lower limit of normal (LLN) and levels of serum IgM 8% below the LLN.
* Absolute CD4+ and CD8+ counts and CD4:CD8 ratio - within normal limits.

Where this trial is running

San Francisco, California

• University of California, San Francisco — San Francisco, California, United States (Recruiting)

Study contacts

• Principal investigator: Joseph Sabatino, MD, PhD — University of California, San Francisco
• Study coordinator: Joseph Sabatino, MD, PhD

Conditions Multiple Sclerosis, Relapsing-Remitting; Ocrelizumab; Lymph node; Lymphocyte