Effects of a nitrate mouth rinse on the oral microbiome

Nitrate Mouth Rinse and the Oral Microbiome

Not applicable Interventional Dalhousie University · NCT06588049

This study is testing if a mouth rinse made from beetroot can change the bacteria in your mouth and improve your overall health for people aged 18 to 40.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment30 (estimated)
Ages18 Years to 40 Years
SexAll
SponsorDalhousie University Academic / other
Locations1 site (Halifax, Nova Scotia)
Trial IDNCT06588049 on ClinicalTrials.gov

What this trial studies

This study investigates the impact of a nitrate-rich mouth rinse, derived from beetroot, on the composition of the oral microbiome. Participants aged 18 to 40 will use either the nitrate mouth rinse or a control water rinse daily for two weeks while maintaining their regular oral hygiene. The study will analyze changes in the oral microbiome using 16s rRNA sequencing and will also measure salivary nitric oxide levels and blood pressure before and after the intervention. The goal is to enhance understanding of how oral hygiene products affect both oral and systemic health.

Who should consider this trial

Good fit: Ideal candidates are healthy adults aged 18 to 40 with no uncontrolled systemic diseases.

Not a fit: Patients with uncontrolled systemic diseases, acute infections, or significant oral health issues may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to improved formulations of oral hygiene products that enhance oral and systemic health.

How similar studies have performed: While the specific approach of using a nitrate mouth rinse is novel, related studies on dietary nitrates and health have shown promising results.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* You are between the ages of 18 and 40. Have no uncontrolled systemic diseases.

Participants with mild chronic conditions such as:

* Asthma
* Type 1 Diabetes Mellitus

Exclusion Criteria:

* You are under the age of 18.
* You have uncontrolled systemic diseases, or acute infections.
* You are taking prescription, antibiotics, probiotics, or antimicrobial mouthrinses.
* You have a documented true allergy (i.e Hives, Swelling of lips/throat) Soy, Nuts, Seeds, Dairy, Egg products, Fish/Shellfish or Wheat.
* The presence of acute or chronic oral conditions including:

  * Herpes Simplex Virus (recent cold sores)
  * Stomatitis
  * Oral Lichen Planus
  * Angular Cheilitis
  * Candidiasis
  * Necrotizing Gingival Diseases
  * Other inflammatory or infectious pathologies
  * Active dental caries "cavities"
  * Any other periodontal diagnosis other than 'Healthy Periodontium' or localized gingivitis

Where this trial is running

Halifax, Nova Scotia

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Nitric OxideOral Microbiome
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.