DNA methylation test for diagnosing thyroid nodules after fine-needle aspiration

Retrospective-Prospective Collection of Thyroid Specimens After FNA

Observational City of Hope Medical Center · NCT03676647

This test tries a DNA methylation signature to detect cancer in people with thyroid nodules who have indeterminate fine-needle aspiration results.

Quick facts

Study typeObservational
Enrollment22 (estimated)
Ages19 Years and up
SexAll
SponsorCity of Hope Medical Center Academic / other
Locations1 site (Duarte, California)
Trial IDNCT03676647 on ClinicalTrials.gov

What this trial studies

This project applies a diagnostic DNA methylation signature (DDMS) to leftover and newly collected fine-needle aspiration (FNA) samples from patients with thyroid nodules to determine whether methylation patterns distinguish benign from malignant lesions. It combines retrospective analysis of previously collected FNA specimens with prospective collection from current patients, analyzing all samples using the DDMS assay. Participants whose samples are tested are followed yearly for five years to track clinical outcomes and confirm diagnoses. The primary goal is to validate the DDMS as a diagnostic tool specifically for nodules with indeterminate cytology (Bethesda III–V).

Who should consider this trial

Good fit: Ideal candidates are people undergoing fine-needle aspiration for thyroid nodules whose cytology is indeterminate (Bethesda category III–V).

Not a fit: Patients with clearly benign or clearly malignant cytology, those who do not undergo FNA, or samples that are insufficient for analysis are unlikely to benefit from this test.

Why it matters

Potential benefit: If successful, the test could reduce unnecessary diagnostic surgery by providing a less invasive way to identify cancer in indeterminate thyroid nodules.

How similar studies have performed: Other molecular classifiers for thyroid nodules (such as genomic expression or mutation panels) have improved diagnostic accuracy, while methylation-based diagnostics are a newer approach with limited but promising supporting data.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* All patients undergoing FNA for thyroid nodule diagnosis.
* Any race and ethnicity are eligible for the study.
* Patient should have thyroid nodule(s) with indeterminate diagnosis after FNA (Bethesda category of indeterminate FNA \[III-V\]).

Where this trial is running

Duarte, California

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Thyroid Gland Nodule
Last reviewed 2026-06-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.