Detecting Microbial DNA/RNA in Patients Undergoing Stem Cell Transplant

Metagenomics Next-Generation Sequencing Approach to Detect Microbial DNA/RNA Overtime in Individuals Undergoing Hematopoietic Stem Cell Transplant

Observational National Institutes of Health Clinical Center (CC) · NCT06904053

This study is testing a new way to find germs in the blood of patients getting stem cell transplants to see if it can help understand infections better.

Quick facts

Study typeObservational
Enrollment30 (estimated)
Ages3 Years to 100 Years
SexAll
SponsorNational Institutes of Health Clinical Center (CC) NIH
Locations1 site (Bethesda, Maryland)
Trial IDNCT06904053 on ClinicalTrials.gov

What this trial studies

This observational study aims to evaluate the effectiveness of metagenomic next-generation sequencing (mNGS) in detecting microbial signatures in patients undergoing hematopoietic stem cell transplantation (HSCT). Participants will provide blood samples before the transplant and for six months afterward, allowing researchers to analyze changes in microbial DNA/RNA over time. The study will also explore correlations between microbial identification and clinical features of infection, such as fever. By comparing mNGS results with routine clinical tests, the study seeks to enhance understanding of infections in immunocompromised patients.

Who should consider this trial

Good fit: Ideal candidates include individuals aged 3 years or older who are co-enrolled in another study at the NIH Clinical Center and are undergoing HSCT.

Not a fit: Patients with conditions that contraindicate participation, as determined by the investigator, may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to improved detection and management of infections in patients undergoing HSCT.

How similar studies have performed: Other studies utilizing metagenomic sequencing approaches have shown promise in detecting infections, suggesting potential success for this novel application.

Eligibility criteria

Show full inclusion / exclusion criteria
* INCLUSION CRITERIA:

In order to be eligible to participate in this study; an individual must meet all the following criteria:

1. Male or female, aged 3 years or older.
2. Co-enrolled on another study at the NIH CC, under which they will undergo HSCT.
3. Stated willingness to comply with all study procedures and availability for the duration of the study.
4. Ability of subject or Legally Authorized Representative (LAR) to understand and the willingness to sign a written informed consent document.

EXCLUSION CRITERIA:

An individual with any condition that, in the opinion of the investigator, contraindicates participation in this study, will be excluded.

Where this trial is running

Bethesda, Maryland

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Hematopoietic Stem Cell TransplantationMicrobial DNA/RNAHematopoietic stem cell transplant
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.