Detecting azole resistance in invasive aspergillosis patients using blood samples

Detection of Azole Resistance Inducing Mutations on DNA Extracted Directly From Serum or Plasma of Immunocompromised Patients With an Invasive Aspergillus Infection Azole Resistance PCR Optimalization-study

Erasmus Medical Center · NCT06069505

This study is testing a new way to quickly find out if patients with a serious fungal infection have a type that is resistant to common treatments, using blood samples from people with weakened immune systems.

Quick facts

Study typeObservational
Enrollment300 (estimated)
Ages18 Years and up
SexAll
SponsorErasmus Medical Center (other)
Locations1 site (Rotterdam, South Holland)
Trial IDNCT06069505 on ClinicalTrials.gov

What this trial studies

This observational study focuses on immunocompromised patients suffering from invasive aspergillosis, a serious fungal infection. The researchers aim to optimize a PCR method to detect mutations associated with azole resistance in fungal DNA extracted directly from serum or plasma. By identifying these mutations quickly, the study seeks to enhance the diagnosis and treatment outcomes for patients infected with azole-resistant Aspergillus fumigatus. The study is particularly relevant given the high prevalence of azole resistance in the Netherlands.

Who should consider this trial

Good fit: Ideal candidates for this study are immunocompromised individuals aged 18 and older with lung lesions indicative of possible invasive fungal infection.

Not a fit: Patients who are unable or unwilling to provide consent will not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to faster and more accurate diagnosis of azole resistance, improving treatment outcomes for patients with invasive aspergillosis.

How similar studies have performed: While the approach of detecting azole resistance mutations is critical, this specific methodology has not been widely tested in similar studies, making it a novel approach.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* 18 years or older.
* Lung CT shows lesions that fulfil the EORTC/MSG radiological criteria of possible invasive fungal infection.
* A bronchoalveolar lavage is planned or has been performed \<48hrs earlier

Exclusion Criteria:

\- Patients unable or unwilling to provide consent

Where this trial is running

Rotterdam, South Holland

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.

View on ClinicalTrials.gov →

Conditions: Invasive Aspergillosis, invasive aspergillosis, PCR

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.