Daytime patterns of breath chemicals in people with and without diabetes

The Daytime Circadian Rhythm in Exhaled Volatile Organic Compounds in People Living Without and With Type 1 and 2 Diabetes: an Exploratory Study

Observational University of Bern · NCT05984979

This study will test whether levels of volatile chemicals in daytime breath follow a circadian pattern in adults with or without type 1 or type 2 diabetes.

Quick facts

Study typeObservational
Enrollment60 (estimated)
Ages18 Years and up
SexAll
SponsorUniversity of Bern Academic / other
Locations1 site (Bern)
Trial IDNCT05984979 on ClinicalTrials.gov

What this trial studies

This observational study will collect repeated daytime breath samples to measure volatile organic compounds (VOCs) and identify circadian variability. Participants are divided into three cohorts—adults without diabetes (HbA1c <6.5%), adults with type 1 diabetes on MDI or CSII for over one year, and adults with type 2 diabetes on oral medication or insulin—with a planned total of 60 participants. A standardized meal is used to reduce dietary variability, and common exclusion criteria include recent smoking, chronic lung or intestinal disease, pregnancy, recent antibiotics, and certain food intolerances. Study data will be compared across groups to detect time-of-day differences in VOC profiles that could inform future biomarker work.

Who should consider this trial

Good fit: Ideal candidates are adults (≥18) who meet one of the three group criteria—no diabetes with HbA1c <6.5%, type 1 diabetes on MDI/CSII >1 year, or type 2 diabetes on oral agents or insulin—and who are non-smokers, not pregnant, and otherwise meet the health eligibility rules.

Not a fit: People who are pregnant or breastfeeding, recent smokers, have chronic lung or intestinal disease, recent antibiotic use, certain food intolerances, heavy alcohol use, or current inhaled medications are unlikely to benefit or be eligible.

Why it matters

Potential benefit: If successful, this could reveal time-of-day breath markers that help monitor metabolic status or guide the timing of tests for people with diabetes.

How similar studies have performed: Prior research indicates breath VOCs can reflect metabolic and inflammatory states, but daytime circadian patterns specifically in people with diabetes are relatively under-studied.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion criteria

Subgroup 1:

* Age 18 or older
* HbA1c below 6.5%
* Written informed consent

Subgroup 2:

* Age 18 or older
* T1D (Type 1 diabetes) with MDI (multiple daily injection) or CSII (Continuous subcutaneous insulin infusion therapy) therapy \>1 year
* Written informed consent

Subgroup 3

* Age 18 or older
* T2D (Type 2 diabetes) with oral antidiabetic medication or insulin therapy
* Written informed consent

Exclusion criteria

* Pregnancy or breastfeeding
* Smoking (last cigarette less than 6 months ago)
* Any chronical lung and intestinal disease diagnosis (such as intestinal bowel disease (IBD); asthma; COPD (chronic obstructive pulmonary disease); lung cancer, …)
* Coeliac disease
* Lactose and fructose intolerance
* Any acute disease diagnosis (such as viral or bacterial infection)
* Drinking habit of more than four units of alcohol per day
* Current inhaled medicines treatments
* Antibiotic treatment in the previous 4 months

Where this trial is running

Bern

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Diabetes Mellitus
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.