Cryopreservation of testicular tissue for fertility preservation

Testicular Tissue Cryopreservation for Fertility Preservation in Patients Facing Infertility-causing Diseases or Treatment Regimens.

Not applicable Interventional University of Colorado, Denver · NCT06387498

This study is testing whether freezing testicular tissue can help men at risk of infertility due to illness or treatment keep their options open for having children in the future.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment60 (estimated)
SexMale
SponsorUniversity of Colorado, Denver Academic / other
Drugs / interventionschemotherapy, radiation, Cyclophosphamide
Locations1 site (Aurora, Colorado)
Trial IDNCT06387498 on ClinicalTrials.gov

What this trial studies

This study focuses on male patients who are at risk of infertility due to disease or gonadotoxic treatments. It aims to evaluate the feasibility and acceptability of testicular tissue cryopreservation as a method for preserving fertility. The study involves the procurement and processing of testicular tissue, followed by cryopreservation, while ensuring safety from malignant cell contamination. The goal is to provide a potential resource for future fertility restoration in patients who currently have no options for preserving their fertility.

Who should consider this trial

Good fit: Ideal candidates include males of any age scheduled for treatments that pose a significant risk of infertility.

Not a fit: Patients who do not face infertility risks from their medical conditions or treatments may not benefit from this study.

Why it matters

Potential benefit: If successful, this approach could offer a viable option for fertility preservation in men facing infertility due to medical treatments.

How similar studies have performed: While animal models and human organ donor experiments have shown promise, rigorous safety and efficacy data in human patients is still lacking, making this approach relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1. Male at any age.
2. Scheduled to undergo surgery, chemotherapy, drug treatment and/or radiation for the treatment or prevention of a medical condition or malignancy with risk of causing permanent and complete loss of subsequent testicular function. Be in significant risk of infertility as defined by:

   * Cyclophosphamide equivalent dose (CED) ≥4 g/m2
   * Total body irradiation (TBI)
   * Testicular radiation \>2.5 Gy
   * Cisplatin 500 mg/m2
   * Bone Marrow Transplant (BMT)
3. Or have a medical condition or malignancy that requires removal of all or part of one or both testicles.
4. Or have newly diagnosed or recurrent disease. Those who were not enrolled at the time of initial diagnosis (i.e., patients with recurrent disease) are eligible if they have not previously received therapy that is viewed as likely to result in complete and permanent loss of testicular function.
5. Have two testicles if undergoing elective removal of a testicle for fertility preservation only. Note: removal of both testicles will limit fertility preservation options.
6. Sign an approved informed consent and authorization permitting the release of personal health information. The patient and/or the patient's legally authorized guardian must acknowledge in writing that consent for specimen collection has been obtained, in accordance with institutional policies approved by the U.S. Department of Health and Human Services.
7. Consent for serum tests for infectious diseases \[including Hepatitis B Surface Antigen, Hepatitis C Virus (HCV) Antibody, and Human Immunodeficiency Virus (HIV) antigen/antibody screen to be performed at the time of testicular tissue harvesting.
8. Undergo a full history and physical examination and obtain standard pre-operative clearance (based on the most recent American College of Cardiology/American Heart Association (ACC/AHA) Guideline for Perioperative Cardiovascular Evaluation for Noncardiac Surgery) as determined by their primary surgeon.

Exclusion Criteria

Patients will be ineligible for participation in this study if they are:

1. Diagnosed with psychological, psychiatric, or other conditions which prevent giving fully informed consent.
2. Diagnosed with an underlying medical condition that significantly increases their risk of complications from anesthesia and surgery.

Where this trial is running

Aurora, Colorado

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Fertility IssuesFertility preservation
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.