Comparing dry versus humidified incubators for sibling oocyte blastocyst development
Impact of Dry vs Humidified Culture Conditions on Blastocyst Development and Aneuploidy: A Time-lapse Sibling Oocyte Study
NA · ART Fertility Clinics LLC · NCT07270250
This test checks whether keeping sibling eggs in a dry versus a humidified GERI time‑lapse incubator changes embryo development for people having PGT‑A with ICSI.
Quick facts
| Phase | NA |
|---|---|
| Study type | Interventional |
| Enrollment | 60 (estimated) |
| Ages | 18 Years to 43 Years |
| Sex | Female |
| Sponsor | ART Fertility Clinics LLC (other) |
| Locations | 1 site (Abu Dhabi, Abu Dhabi Emirate) |
| Trial ID | NCT07270250 on ClinicalTrials.gov |
What this trial studies
This interventional sibling‑oocyte study splits oocytes from the same cycle between dry and humidified chambers of a GERI time‑lapse incubator under continuous culture conditions. Embryos are monitored by time‑lapse imaging and cultured to blastocyst stage, with outcomes recorded such as total blastulation, blastocyst quality, biopsy rate, fertilization rate, and aneuploidy after PGT‑A. The design compares matched sibling oocytes to control for patient‑level factors and isolate the effect of incubation humidity. Results aim to inform whether one chamber type yields better developmental outcomes while supporting uninterrupted lab workflows.
Who should consider this trial
Good fit: Ideal candidates are people aged 18–43 undergoing PGT‑A with ICSI who expect multiple mature oocytes (≥4), have BMI <35, and will use fresh or frozen ejaculated sperm.
Not a fit: People using PGT‑M or PGT‑SR, those using testicular sperm, undergoing conventional IVF insemination, or with a history of fertilization failure are excluded and unlikely to benefit from this protocol.
Why it matters
Potential benefit: If successful, the trial could identify an incubation approach that improves blastocyst development and helps labs choose methods that may raise IVF success while streamlining workflow and costs.
How similar studies have performed: Some small reports suggest humidified continuous‑culture conditions may improve blastocyst development in no‑refresh systems, but existing data are limited and inconsistent.
Eligibility criteria
Show full inclusion / exclusion criteria
Inclusion Criteria: * PGT-A cycles with more than at least 4 mature oocytes * ICSI * maternal age 18- 43 years old * PGT-A intended cycles with trophectoderm biopsies on day 5 /6/7 * patients with more than 8 oocytes expected for ICSI * BMI \<35 * fresh and frozen ejaculated sperm Exclusion Criteria: * PGT-M /PGT-SR cycles * fresh and frozen testicular sperm * IVF insemination * previous history of fertilization failure
Where this trial is running
Abu Dhabi, Abu Dhabi Emirate
- ART Fertility Clinics LLC — Abu Dhabi, Abu Dhabi Emirate, United Arab Emirates (RECRUITING)
Study contacts
- Study coordinator: JONALYN EDADES, EMBA Healthcare Management
- Email: jonalyn.edades@artfertilityclinics.com
- Phone: +97126528000
How to participate
- Review the eligibility criteria above with your treating physician.
- Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
- Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions: Incubator Stay, Total Blastulation, Blastocyst Quality, Biopsy Rate, Fertilization Rate, Ploidy Rate, ICSI