Comparing DNA sequencing and culturing methods for diagnosing infectious keratitis
Evaluation of Next-Generation Sequencing-based Metabarcoding Versus Culturing in Infectious Keratitis
This study is testing whether a new DNA sequencing method is better than traditional culturing for finding the germs that cause infections in the eye for people with corneal issues.
Quick facts
| Study type | Observational |
|---|---|
| Enrollment | 100 (estimated) |
| Ages | 18 Years and up |
| Sex | All |
| Sponsor | Aarhus University Hospital Academic / other |
| Locations | 1 site (Aarhus) |
| Trial ID | NCT05183906 on ClinicalTrials.gov |
What this trial studies
This study evaluates the effectiveness of Next Generation Sequencing (NGS) compared to traditional culturing methods for identifying the microorganisms responsible for infectious keratitis. Patients with a corneal infiltrate are assessed at Aarhus University Hospital using both techniques to determine which method provides more accurate results. The study includes sampling from both the affected eye and the contralateral healthy eye to analyze the normal microbiome. A total of 100 patients will be enrolled to ensure robust data collection.
Who should consider this trial
Good fit: Ideal candidates are patients aged 18 and older with a corneal infiltrate of at least 2 mm or 1 mm within the central 6 mm of the cornea.
Not a fit: Patients under 18 years of age or those without significant corneal infiltrates will not benefit from this study.
Why it matters
Potential benefit: If successful, this study could lead to more accurate and timely diagnoses of infectious keratitis, potentially improving patient outcomes and preserving vision.
How similar studies have performed: While the use of NGS in microbiological assessments is gaining traction, this specific comparison with culturing methods in the context of infectious keratitis is relatively novel.
Eligibility criteria
Show full inclusion / exclusion criteria
Inclusion Criteria: - Patients with a corneal infiltrate of minimum 2 mm in diameter or of 1 mm diameter within the central 6 mm of the cornea Exclusion Criteria: - Patients below 18 years of age
Where this trial is running
Aarhus
- Department of Ophthalmology, Aarhus University Hospital — Aarhus, Denmark (Recruiting)
How to participate
- Review the eligibility criteria above with your treating physician.
- Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
- Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.