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Combining trametinib and azacitidine for treating juvenile myelomonocytic leukemia

Risk Stratified Treatment for Patients With Newly Diagnosed Juvenile Myelomonocytic Leukemia: A Phase I/II Non-randomized Study of Trametinib and Azacitidine With or Without Chemotherapy (IND #164058)

PHASE1; PHASE2 · Therapeutic Advances in Childhood Leukemia Consortium · NCT05849662

This study is testing a new combination of medications to see if they can safely help children with juvenile myelomonocytic leukemia feel better, depending on their risk level.

Quick facts

PhasePHASE1; PHASE2
Study typeInterventional
Enrollment58 (estimated)
Ages1 Month to 21 Years
SexAll
SponsorTherapeutic Advances in Childhood Leukemia Consortium (other)
Drugs / interventionstrametinib, chemotherapy, immunotherapy, radiation, methotrexate, fludarabine
Locations19 sites (Phoenix, Arizona and 18 other locations)
Trial IDNCT05849662 on ClinicalTrials.gov

What this trial studies

This clinical trial aims to evaluate the safety and efficacy of trametinib in combination with azacitidine for patients with newly diagnosed juvenile myelomonocytic leukemia (JMML). Lower-risk JMML patients will receive trametinib and azacitidine, while high-risk patients will receive a combination of trametinib, azacitidine, fludarabine, and cytarabine. The study will assess the safety of these drug combinations over multiple treatment courses, with specific dosing regimens tailored to the risk level of the patients.

Who should consider this trial

Good fit: Ideal candidates are children and young adults aged 1 month to 21 years with newly diagnosed JMML meeting specific clinical and genetic criteria.

Not a fit: Patients who do not meet the diagnostic criteria for JMML or are older than 21 years may not benefit from this study.

Why it matters

Potential benefit: If successful, this treatment approach could improve outcomes for children diagnosed with juvenile myelomonocytic leukemia.

How similar studies have performed: While this approach is novel in the context of JMML, similar combinations have shown promise in other hematological malignancies.

Eligibility criteria

Show full inclusion / exclusion criteria 
Inclusion Criteria:

Age

• Patients must be ≥ 1 month and ≤21 years of age at enrollment.

Diagnosis • Patients must meet the 2022 International Consensus Classification criteria for JMML. The diagnosis is made based on the following criteria:.

Clinical and hematologic features (the first 2 features are present in most cases; the last 2 are required):

* Peripheral blood monocyte count ≥ 1 × 109/L\*
* Splenomegaly†
* Blast percentage in PB and BM \< 20%
* Absence of BCR::ABL1

  * This monocyte threshold is not reached in approximately 7% of cases. †Splenomegaly is absent in 3% of cases at presentation.

II. Genetic studies (1 finding required):

* Somatic mutation in PTPN11‡ or KRAS‡ or NRAS‡ or RRAS or RRAS2‡
* Clinical diagnosis of neurofibromatosis type 1 or germline NF1 mutation and loss of heterozygosity of NF1 or somatic biallelic loss of NF1
* Germline CBL mutation and loss of heterozygosity of CBL, or somatic mutation(s) in CBL§

  * Germline mutations (indicating Noonan syndrome) need to be excluded. §Occasional cases with heterozygous splice site mutations.

Performance Level

* Karnofsky \> 50% for patients ≥ 16 years of age
* Lansky \> 50% for patients \< 16 years of age.

Prior Therapy

* No prior leukemia directed therapy is permitted with the exception of:

  1. Cytoreduction with hydroxyurea can be initiated and continued for up to 24 hours prior to the start of trametinib.
  2. Cytoreduction with 6-mercaptopurine (6-MP) 6-MP can be initiated and continued for up to 72 hours prior to the start of trametinib.
  3. Intrathecal (IT) cytarabine, IT methotrexate or triple IT therapy (cytarabine, methotrexate and hydrocortisone) within 7 days of enrollment as part of a diagnostic evaluation.

     No prior hematopoietic stem cell transplant is permitted.

     Adequate Renal Function Defined as:
* Patient must have a calculated creatinine clearance or radioisotope GFR ≥ 70ml/min/1.73m2 OR a normal serum creatinine based on age/gender in the chart below:

Maximum Serum Creatinine (mg/dL):

* 1 month to \< 6 months old - Male: 0.4, Female 0.4
* 6 months to \<1 year old - Male 0.5, Female 0.5
* 1 to \< 2 years old - Male: 0.6, Female: 0.6
* 2 to \< 6 years old - Male:0.8, Female: 0.8
* 6 to \< 10 years old - Male: 1, Female: 1
* 10 to \< 13 years old - Male: 1.2, Female: 1.2
* 13 to \< 16 years old - Male: 1.5, Female: 1.4
* ≥ 16 years old - Male: 1.7, Female: 1.4 The threshold creatinine values in this Table were derived from the Schwartz formula for estimating GFR (Schwartz et al. J. Peds, 106:522, 1985) utilizing child length and stature data published by the CDC.

Adequate Liver Function Defined as:

* Direct bilirubin \< 1.5 x upper limit of normal (ULN) for age or normal, AND alanine transaminase (ALT) \< 5 x ULN for age.
* The hepatic requirements are waived for patients with known or suspected liver involvement by leukemia and will not be evaluable for hepatotoxicity. This must be reviewed and approved by the study chair or vice chair.

Adequate Cardiac Function Defined as:

* Ejection fraction of \> or = to 50% by echocardiogram, OR
* Ejection fraction of \> or = to 50% by radionuclide angiogram (MUGA).

Reproductive Function

* Female patients of childbearing potential must have a negative urine or serum pregnancy test confirmed within 2 weeks prior to enrollment.
* Female patients with infants must agree not to breastfeed their infants while on this study.
* Male and female patients of child-bearing potential must agree to use an effective method of contraception approved by the investigator during the study and for a minimum of 6 months after study treatment.

Exclusion Criteria:

* Patients cannot have a known allergy to any of the drugs used in the study.
* Patients cannot have a systemic fungal, bacterial, viral, or other infection that is exhibiting ongoing signs/symptoms related to the infection without improvement despite appropriate antibiotics or other treatment. The patient needs to be off pressors and have negative blood cultures for 48 hours.
* Patients cannot have a plan to administer non-protocol chemotherapy, radiation therapy, or immunotherapy during the study period.
* Patients cannot have significant concurrent disease, illness, psychiatric disorder or social issue that would compromise patient safety or compliance with the protocol treatment or procedures, interfere with consent, study participation, follow up, or interpretation of study results.
* Patients cannot have a clinical or molecular diagnosis of Noonan syndrome. Note: patients with either neurofibromatosis type 1 or Casitas B-lineage lymphoma (CBL) syndrome (also known as Noonan-like syndrome), are eligible to enroll. Patients with Down syndrome are excluded from the study.
* Patient cannot have had prior use of hematopoietic growth factors, biologics (anti-neoplastic agent), or XRT.
* Patients cannot be taking any medications for treatment of left ventricular systolic dysfunction.
* Patients cannot have a history of or current evidence of retinal vein occlusion (RVO) or central serous retinopathy (CSR).
* Patients cannot have had prior use of any MEK inhibitor.

Where this trial is running

Phoenix, Arizona and 18 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.

View on ClinicalTrials.gov →

Conditions: Leukemia, Juvenile Myelomonocytic, JMML, JCML, Neurofibromatosis 1, CBL Syndrome

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.