Characterizing T-cell responses to arbovirus infections using ELISpot tests
ARBOspot: Characterization of Arbovirus Specific T Cell Response in Patients by Using Enzyme Linked Immunospot (ELISpot) Assays
Fondazione IRCCS Policlinico San Matteo di Pavia · NCT07291206
This project will test whether ELISpot blood assays can detect T-cell immune responses in adults with suspected arbovirus infections.
Quick facts
| Study type | Observational |
|---|---|
| Enrollment | 500 (estimated) |
| Ages | 18 Years and up |
| Sex | All |
| Sponsor | Fondazione IRCCS Policlinico San Matteo di Pavia (other) |
| Locations | 1 site (Pavia, Lombardy) |
| Trial ID | NCT07291206 on ClinicalTrials.gov |
What this trial studies
This observational project will enroll adults with suspected arbovirus infection at Fondazione IRCCS Policlinico San Matteo in Pavia and collect blood samples for immunological analysis. Researchers will use ELISpot and related assays to measure virus-specific T-cell responses and compare results with serology and clinical information. The work will include endemic and imported arboviruses such as WNV, TOSV, SFSV, SFNV, USUV, TBEV, CHIKV, DENV, ZIKV, YFV, and JEV. Results aim to map cellular immune profiles that could inform diagnosis and surveillance strategies.
Who should consider this trial
Good fit: Adults aged 18 or older of any sex with a suspected arbovirus infection who can give written informed consent are ideal candidates.
Not a fit: People without a current suspected arbovirus infection or those with conditions that would compromise safety or interfere with immune testing are unlikely to benefit.
Why it matters
Potential benefit: If successful, this could improve understanding of protective immune responses and help refine diagnostic and surveillance tools for arbovirus infections.
How similar studies have performed: ELISpot and similar T-cell assays have been used successfully to detect cellular immune responses in other viral infections and in some arbovirus research.
Eligibility criteria
Show full inclusion / exclusion criteria
Inclusion Criteria: * Adults 18 years- old and over; all sexes will be eligible for the study; * Suspected arbovirus infection * Written informed consent and any required authorization obtained from the participant prior to performing any protocol-related procedures; Exclusion Criteria: * Any condition that might compromise safety or interfere with study results. * All other conditions not present in the inclusion criteria.
Where this trial is running
Pavia, Lombardy
- Fondazione IRCCS Policlinico San Matteo — Pavia, Lombardy, Italy (RECRUITING)
Study contacts
- Study coordinator: Baldanti
- Email: segreteria.virologia@smatteo.pv.it
- Phone: +390382502420
How to participate
- Review the eligibility criteria above with your treating physician.
- Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
- Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions: Arbovirus Infections, ELISpot, immune response, serology