Characterizing asthma severity through gene expression analysis

Phenotyping Disease Severity in Asthma: Molecular Investigations of Corticosteroid

University of Calgary · NCT05078021

This study is trying to see how gene activity in the lungs of asthma patients with different severity levels responds to treatment compared to healthy individuals.

Quick facts

Study typeObservational
Enrollment60 (estimated)
Ages18 Years to 60 Years
SexAll
SponsorUniversity of Calgary (other)
Drugs / interventionsprednisone
Locations1 site (Calgary, Alberta)
Trial IDNCT05078021 on ClinicalTrials.gov

What this trial studies

This observational study aims to analyze and compare the molecular gene expression profiles in endobronchial biopsies and bronchial washings from asthma patients with varying severity levels who are on maintenance inhaled corticosteroid treatment, alongside healthy controls. By recruiting 60 participants, including 15 each from mild, moderate, and severe asthma categories as defined by GINA guidelines, the study will produce transcriptomic profiles associated with asthma severity. Additionally, the study will culture epithelial cells to investigate differences in corticosteroid responses between the groups, potentially identifying intrinsic differences in treatment efficacy.

Who should consider this trial

Good fit: Ideal candidates include adults aged 18-60 with a confirmed diagnosis of asthma and stable inhaled therapy for at least 12 weeks.

Not a fit: Patients who are current smokers or have a significant smoking history may not benefit from this study.

Why it matters

Potential benefit: If successful, this study could lead to improved understanding of asthma severity and more tailored treatment approaches for patients.

How similar studies have performed: While there have been studies on gene expression in blood, this approach focusing on airway transcriptomics in asthma severity is relatively novel.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Age 18-60
* Confirmed diagnosis of asthma by CTS criteria
* No contraindication to bronchoscopy
* No treatment with azithromycin
* No oral corticosteroid in the 4 weeks prior
* No participation in another drug study in the 4 weeks prior
* On stable doses of asthma inhaled therapies for 12 weeks prior to bronchoscopy
* FEV1 \>80%

Exclusion Criteria:

* Current smokers (within past year)
* Subjects with ≥10 pack-year lifetime smoking history
* History of asthma exacerbation (requiring oral prednisone) in the 4 weeks prior to study entry

Where this trial is running

Calgary, Alberta

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.

View on ClinicalTrials.gov →

Conditions: Asthma

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.