Causes of unexplained fevers in Nepal (DEFINe)

Determination of Etiology of Febrile Illness in Nepal (DEFINe Study)

Not applicable Interventional Patan Academy of Health Sciences · NCT07004751

This project will test whether adding cultures and ELISA blood tests can find the causes of fever and reduce unnecessary antibiotic use in adults admitted with 3–21 day unexplained fevers in Nepal.

Quick facts

PhaseNot applicable
Study typeInterventional
Enrollment194 (estimated)
Ages16 Years and up
SexAll
SponsorPatan Academy of Health Sciences Academic / other
Locations1 site (Lalitpur, Bagmati)
Trial IDNCT07004751 on ClinicalTrials.gov

What this trial studies

This hospital-based interventional effort enrolls adults with documented fevers of 3 to 21 days and no clear clinical, radiological, or routine laboratory diagnosis. Participants receive additional microbiological testing beyond local standard of care, including culture techniques and ELISA serology for seven pathogens not routinely tested in Nepal. The study compares etiologic detection and subsequent antimicrobial use when these additional tests are applied. Results will inform whether broader diagnostic testing changes treatment decisions and identifies otherwise-missed causes of febrile illness.

Who should consider this trial

Good fit: Adults aged 16 years or older admitted to the medical unit at Patan Academy with documented fever lasting 3–21 days, no obvious diagnosis after initial testing, and who can provide informed consent.

Not a fit: Outpatients, children under 16, immunocompromised individuals (including known HIV or ANC <500), patients with fevers shorter than 3 days or longer than 21 days, those being discharged from the emergency room, or recently hospitalized patients are not expected to benefit from this protocol.

Why it matters

Potential benefit: If successful, this could let clinicians target treatment more accurately, reduce unnecessary antibiotic use and costs, and help slow antibiotic resistance.

How similar studies have performed: Previous regional studies have shown that adding microbiological testing can identify more causes of fever and reduce empirical antibiotic use, though this specific panel of tests has not been widely implemented in Nepal.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Age 16 years or older
* Documented fever (T ≥100.4F or ≥38C) of 3 days to 21 days duration
* No obvious diagnosis found on the basis of clinical, radiological, or initial routine laboratory tests\*
* Admitted to the medical unit of hospital
* Signed ICF (informed consent form)

Exclusion Criteria:

* Outpatients
* Fever of less than 3 days or more than 21 days duration
* Patients being discharged from Emergency Room
* Children (younger than 16 years in age)\*\*
* Immunocompromised patients
* Neutropenia (ANC \<500 per cumm)
* Known case of HIV infection
* Admission to a hospital for 48-hours or longer within past 30 days

Where this trial is running

Lalitpur, Bagmati

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Febrile IllnessFever Without SourceFebrile Illnes
Last reviewed 2026-06-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.