CAR T-cell therapy for relapsed B-lineage leukemia and lymphoma

CD19-Directed Chimeric Antigen Receptor (CAR) T-Cell Therapy for Relapsed/Refractory B-Lineage Leukaemia / Lymphoma - A Feasibility Protocol

Phase 2 Interventional KK Women's and Children's Hospital · NCT05648019

This study is testing if a new CAR T-cell therapy can be safely given to patients with relapsed B-lineage leukemia and lymphoma to see how well it works.

Quick facts

PhasePhase 2
Study typeInterventional
Enrollment40 (estimated)
Ages0 Years to 70 Years
SexAll
SponsorKK Women's and Children's Hospital Government
Drugs / interventionsCAR T
Locations2 sites (Singapore and 1 other locations)
Trial IDNCT05648019 on ClinicalTrials.gov

What this trial studies

This study evaluates the feasibility of delivering CD19-directed CAR T-cell therapy to patients with relapsed or refractory B-lineage leukemia and lymphoma. It is a single-arm, open-label, multi-center phase II study that includes several phases: screening for eligibility, a preparatory phase involving leukapheresis and CAR T-cell manufacturing, a treatment phase with a single dose infusion of CAR T-cells, and a follow-up phase for monitoring efficacy and safety for up to 24 months. The goal is to assess how well this therapy can be administered in a point-of-care setting.

Who should consider this trial

Good fit: Ideal candidates include patients under 18 years or adults with relapsed or refractory B-cell acute lymphoblastic leukemia or large B-cell lymphoma who have undergone multiple lines of systemic therapy.

Not a fit: Patients with non-B-lineage leukemias or lymphomas, or those who are not relapsed or refractory, may not benefit from this study.

Why it matters

Potential benefit: If successful, this therapy could provide a new treatment option for patients with difficult-to-treat relapsed or refractory B-lineage leukemia and lymphoma.

How similar studies have performed: Other studies using CAR T-cell therapy for similar conditions have shown promising results, indicating that this approach is gaining traction in the treatment of hematological malignancies.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

1. Eligible disease conditions:

   1. Relapsed or refractory B-cell ALL (all must be satisfied)

      * Presence of lymphoblasts in bone marrow aspirate by morphologic assessment or positive minimal residual disease at screening.
      * Relapsed or refractory or ineligible for HSCT
      * For relapsed B-ALL: Documentation of CD19 tumour expression (e.g. by flow cytometry) demonstrated in bone marrow or peripheral blood within 3 months of study entry
   2. Relapsed or refractory large B-cell lymphoma after two or more lines of systemic therapy, including diffuse large B-cell lymphoma (DLBCL) not otherwise specified, primary mediastinal large B-cell lymphoma, high grade B-cell lymphoma, and DLBCL arising from follicular lymphoma.
2. Age at screening:

   1. \< 18 years (paediatric group); or
   2. ≥ 18 years (adult group)
3. Adequate organ functions:
4. Life expectancy more than 12 weeks.
5. Karnofsky (age ≥ 16 years) or Lansky (age \< 16 years) performance status ≥ 50 at screening.
6. Must meet the institutional criteria to undergo leukapheresis or have a leukapheresis product of non-mobilized cells received and accepted by the manufacturing site.

Exclusion Criteria:

Patients with any of the following will be excluded:

* B-ALL with isolated extramedullary disease relapse
* Patients with concomitant genetic syndrome: such as patients with Fanconi anaemia, Kostmann syndrome, Shwachman syndrome or any other known bone marrow failure syndrome. Patients with Down syndrome will not be excluded.
* Patients with Burkitt's lymphoma/leukaemia (i.e. patients with mature B-cell ALL; leukaemia with B-cell \[sIg positive and kappa or lambda restricted positivity\] ALL, with FAB L3 morphology and /or a MYC translocation)
* Active or latent hepatitis B or active hepatitis C (test within 8 weeks of screening), or any uncontrolled infection at screening
* Human Immunodeficiency Virus (HIV) positive test within 8 weeks of screening
* Presence of grade 2 to 4 acute or extensive chronic graft-versus-host disease (GVHD)
* Active CNS involvement by malignancy, defined by CNS-3 per NCCN guidelines. Subjects with CNS-2 involvement or with history of CNS disease that have been actively treated are eligible.
* Patient has an investigational medicinal product within the last 30 days prior to screening.
* Pregnant or nursing women.
* Women of childbearing potential (defined as all women physiologically capable of becoming pregnant) and all male participants, unless they are using highly effective methods of contraception for a period of 1 year after the CAR T-cell infusion. All female patients of childbearing potential must have a negative pregnancy test performed within 48 hours before infusion of CAR T-cells.

The following are not strictly exclusion criteria but must be discussed with PI/Site-PI:

* Prior malignancy, except carcinoma in situ of the skin or cervix treated with curative intent and with no evidence of active disease
* Treatment with any prior gene therapy product
* Has had treatment with any prior anti-CD19/anti-CD3 therapy, or any other anti-CD19 therapy

Where this trial is running

Singapore and 1 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Lymphoblastic LeukemiaLymphoblastic Leukemia in ChildrenLymphoblastic Leukemia, Acute AdultB-cell Acute Lymphoblastic LeukemiaLarge B-cell LymphomaCARCAR T-cell therapyRelapse/Refractory
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.