ATP7B mRNA (DSL101) therapy for Wilson disease

A Clinical Study on the Treatment of Wilson Disease With ATP7B mRNA/LNP (DSL101)

This treatment will test whether one or more IV infusions of ATP7B mRNA (DSL101) are safe and help control copper in adults with genetically confirmed Wilson disease.

Quick facts

• Phase: Early Phase 1
• Study type: Interventional
• Enrollment: 18 (estimated)
• Ages: 18 Years and up
• Sex: All
• Sponsor: DSciLab Co., Ltd. (Industry-sponsored)
• Locations: 1 site (Hefei, Anhui)
• Trial ID: NCT07240896 on ClinicalTrials.gov

What this trial studies

In this open-label, single-arm, non-randomized dose-escalation trial adults with genetically confirmed Wilson disease receive single or multiple intravenous infusions of DSL101 (ATP7B mRNA in LNP) at low, medium, or high dose levels. The low-dose cohort uses an accelerated titration design while the medium and high cohorts use a traditional 3+3 design combined with sentinel dosing to escalate doses. Outcomes include safety, tolerability, preliminary efficacy, pharmacokinetics, and immunogenicity measured over planned follow-up visits. The study is conducted at a single center in Hefei, Anhui, with intensive monitoring around dosing.

Who should consider this trial

Good fit: Adults (≥18 years) with genetically confirmed biallelic ATP7B mutations, a Leipzig score ≥4 and ceruloplasmin <0.1 g/L who have been on a stable standard-of-care regimen and low-copper diet for at least six months and agree to contraception are the intended participants.

Not a fit: Patients without confirmed ATP7B mutations, those with unstable disease or recent treatment changes, pregnant people, or those unable to follow diet or contraception requirements are unlikely to benefit from participation.

Why it matters

Potential benefit: If successful, DSL101 could restore ATP7B protein function, reduce copper accumulation, and improve liver and neurological outcomes for people with Wilson disease.

How similar studies have performed: mRNA replacement for ATP7B is a largely novel approach for Wilson disease with encouraging preclinical results but very limited human data so far.

Eligibility criteria

Inclusion Criteria:

1. Age ≥18 years old, gender not limited.
2. Meet the diagnostic criteria f Wilson's Disease in "Guidelines for Diagnosis and Treatment of Wilson's Disease (2022 Edition)", with a Leipzig score ≥4, at least one year between diagnosis and screening; ceruloplasmin level \<0.1g/L.
3. Patinets with Wilson's disease confirmed by laboratory tests to have double-chromosome mutations in the ATP7B gene.
4. Low copper diet for at least six months befoer screening and willing to continue low copper diet during study.
5. Fertile subjects agreed to adopt reliable contrraceptive methods from the screening until 6 months after the last administration.
6. The subjects are atable patients with WD who have been trasted for at least six months without drug or dose changes for at least 6 momths at the time of screening , and have continuously used standard treatments \[SOC, such as D-penicillamine, sodiu dihydroxypropane sulfonate, dimercaptosuccinic acid, trientine, and zinc preparations (zinc acetate, zinc gluconate, zinc sulfate)\] for at least 6 months screening, and allowed subjects to continue with their prior SOC treatment.
7. The subject's condition was fully controlled after treatment, and its definition must meet all of the following conditions:

   1. Serum NCC level ≥ 25 μg/L and ≤ 150 μg/L;
   2. Urinary copper ≥100 μg/24 hours and ≤900 μg/24 hours;;
   3. ALT \< 2 times of upper limit of normal value (ULN);
   4. The investigator believes that no other laboratory values or clinical symptoms would stop current standard therapy;
8. Subjects with good compliance, who can understand and cooperate to complete the requirements of protocol.
9. The subjects voluntarily participated in the trial and signed the informed consent form.

Exclusion Criteria:

1. Allergy or intolerance to the investigational drug.
2. Wilson's disease is accompanied by severe complications such as neurological and mental disorders.
3. History of liver transplantation.
4. Other liver-related diseases and clinical symptoms that can cause liver injury, such as acute and chronic hepatitis, alcoholic liver disease, autoimmune liver disease, drug-induced liver injury, liver cirrhosis, liver ascites, esophageal varices, hepatic encephalopathy, hepatorenal syndrome, liver failure, liver malignancy, etc.; Subjects with Model for end-stage liver disease score (MELD)\>13.
5. Other diseases that can cause hemolysis or anemia, such as erythrocytosis, Mediterranean anemia, hemolytic anemia, various causes of infection, large area burns, etc.
6. Other diseases that can cause dysfunction of the nervous system, such as Parkinson's disease, Parkinson syndrome, various causes of dystonia, chorea, primary tremor, epilepsy, mental abnormalities (such as history of schizophrenia or suicide attempts), etc.
7. Screening period laboratory examination indicators:

   1. Hemoglobin \< 90 g/L;
   2. Creatinine clearance ≤30 mL/min, or glomerular filtration rate \<45 mL/min/1.73 m²;
   3. TBil≥2×ULN，ALP/TBil\<4，AST/ALT\>2.2;
   4. Platelets \< 70 ×10\^9/L;
   5. Neutrophils \< 1.0 × 10\^9 /L.
8. History of gastrointestinal bleeding within six months before screening.
9. Subjects with history of moderate to severe depression, suicidal thoughts or behaviors and serious psychiatric within 6 months prior to screening.
10. Subjects who have uncontrolled diseases of thr heart, liver, kidneys, endocrine system, digestive tract, metabolism, blood, or malignant tumors.
11. Active hepatitis B virus infection or active hepatitis C virus infection, or human immunodeficiency virus antibody positive.
12. Pregnant women or lactating women.
13. Subjects who have participated other clinical trial within 3 months prior to screening or plan to participate during the clinical trial.
14. Investigators evaluate other subjects who are not suitable to participate in this clinical trial.

Where this trial is running

Hefei, Anhui

• The First Affiliated Hospital of Anhui Medical University — Hefei, Anhui, China (Recruiting)

Conditions Wilsons Disease