Analyzing blood samples to diagnose myelodysplastic syndromes without bone marrow aspiration

Flow Cytometric Analysis of Peripheral Blood Neutrophil Myeloperoxidase Expression for Ruling Out Myelodysplastic Syndromes: Protocol for a Diagnostic Accuracy Study

Observational University Hospital, Grenoble · NCT05175469

This study is testing a new blood test to see if it can accurately diagnose myelodysplastic syndromes without the need for a painful bone marrow procedure.

Quick facts

Study typeObservational
Enrollment400 (estimated)
Ages18 Years to 100 Years
SexAll
SponsorUniversity Hospital, Grenoble Academic / other
Locations8 sites (Clermont-Ferrand and 7 other locations)
Trial IDNCT05175469 on ClinicalTrials.gov

What this trial studies

This study aims to evaluate the accuracy of flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood as a non-invasive diagnostic tool for myelodysplastic syndromes (MDS). It involves a multicenter approach where consecutive patients referred for suspicion of MDS will undergo this blood test, which will be compared against traditional bone marrow examination results. The primary goal is to determine how effectively this blood test can rule out MDS, potentially reducing unnecessary invasive procedures. Secondary objectives include assessing the negative predictive value of the test and identifying alternative diagnoses in patients ruled out for MDS.

Who should consider this trial

Good fit: Ideal candidates are adults aged 18 and older who are referred for suspicion of myelodysplastic syndrome and have indications for bone marrow examination.

Not a fit: Patients with a history of or active myelodysplastic syndromes or chronic myelomonocytic leukemia will not benefit from this study.

Why it matters

Potential benefit: If successful, this approach could significantly reduce the need for invasive bone marrow aspirations in patients suspected of having MDS.

How similar studies have performed: While this approach is innovative, similar studies have not been widely reported, indicating that this may be a novel method for diagnosing MDS.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Age at enrollment ≥18 years
* Referral for suspicion of myelodysplastic syndrome
* Indication for bone marrow examination
* ≥1 peripheral blood cytopenia defined by hemoglobin concentration \<12 g/dL for female and \<13g/dL for male patients, platelet count \<150 x109/L, absolute neutrophil count \<1.8 x109/L
* Inpatient or outpatient care

Exclusion Criteria:

* Refusal to participate
* History of or active documented MDS or CMML
* Enrollment in intensive or critical care unit
* Incarcerated or individuals protected by French regulation (Article L1121.5 and following, Code de la Santé Publique)
* Not affiliated with social security system
* Previous enrollment in the study

Where this trial is running

Clermont-Ferrand and 7 other locations

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Myelodysplastic SyndromesMyelodysplastic syndromesDiagnostic accuracy studyFlow cytometry analysisMyeloperoxidasePeripheral blood sampleAdditional relevant MeSH terms: Preleukemia, Syndrome, Disease, Pathologic Processes
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.