Adding platelet-rich plasma (PRP) to donor sperm during freezing
Effect of Platelet-rich Plasma (PRP) on the Quality of Donor Semen During Cryopreservation
Instituto Valenciano de Infertilidad, IVI Alicante · NCT07504575
This study will try adding platelet-rich plasma (PRP) to sperm during freezing to see if it improves motility after thawing for men providing samples.
Quick facts
| Study type | Observational |
|---|---|
| Enrollment | 20 (estimated) |
| Ages | 18 Years to 35 Years |
| Sex | Male |
| Sponsor | Instituto Valenciano de Infertilidad, IVI Alicante (other) |
| Locations | 1 site (Alicante, Alicante) |
| Trial ID | NCT07504575 on ClinicalTrials.gov |
What this trial studies
This observational study will compare thawed semen samples frozen with differing concentrations of autologous PRP (including 1%, 2% and a primary 5% concentration) against control samples without PRP. The primary outcome is a difference in progressive motility between control and samples frozen with 5% PRP, with secondary analyses of other semen quality parameters and correlations with PRP platelet counts. Semen samples meeting inclusion criteria will be processed, frozen, thawed, and analyzed using standard laboratory measures of motility, viability, morphology, and membrane integrity. The study focuses on ex vivo modifications of the freezing medium rather than clinical pregnancy outcomes.
Who should consider this trial
Good fit: Men aged 18–35 with semen showing >50 million progressively motile sperm and >4% normal morphology, without personal or hereditary pathology or psychological/behavioral disorders, are ideal candidates.
Not a fit: Men with semen parameters below the inclusion thresholds, with relevant medical or hereditary conditions, or outside the specified age range are unlikely to benefit from participation.
Why it matters
Potential benefit: If successful, adding PRP to the freezing medium could improve post-thaw sperm motility and overall semen quality, potentially helping assisted reproduction outcomes.
How similar studies have performed: Previous small studies have reported that PRP components can improve post-thaw motility, viability, and membrane integrity, but the evidence is limited and not yet definitive.
Eligibility criteria
Show full inclusion / exclusion criteria
Inclusion Criteria: Age from 18 to 35 years Concentration higher than 50 million progressively motile sperm. Morphology: more than 4% normal sperm shapes. Exclusion Criteria: Presence of personal or hereditary pathology. Presence of psychological or behavioral disorder. Semen parameters below those indicated in the inclusion criteria.
Where this trial is running
Alicante, Alicante
- IVI Alicante — Alicante, Alicante, Spain (RECRUITING)
Study contacts
- Study coordinator: Elena Sellés, PhD
- Email: elena.selles@ivirma.com
- Phone: +34 966012490
How to participate
- Review the eligibility criteria above with your treating physician.
- Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
- Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions: Sperm Cryodamage, Sperm, PRP, Cryopreservation