360-degree mouth-cleaner manual toothbrush versus standard flat-trim manual toothbrush for reducing mouth bacteria

Evaluate the Effectiveness of Whole Mouth Bacterial Reduction for a Manual Toothbrush With a 360-Degree Mouth Cleaner Design Versus a Standard Flat-Trim Manual Toothbrush.

Phase 3 Interventional Colgate Palmolive · NCT07471477

This test looks at whether a 360-degree mouth-cleaner manual toothbrush reduces whole-mouth bacteria more than a standard flat-trim manual toothbrush in adults 18–65 with at least 20 natural teeth.

Quick facts

PhasePhase 3
Study typeInterventional
Enrollment60 (estimated)
Ages18 Years to 65 Years
SexAll
SponsorColgate Palmolive Industry-sponsored
Locations1 site (Heredia)
Trial IDNCT07471477 on ClinicalTrials.gov

What this trial studies

Adult participants in good general and oral health are assigned to use either a 360-degree mouth-cleaner manual toothbrush (test) or a standard flat-trim manual toothbrush (control), both with 0.76% sodium monofluorophosphate (1000 ppm F). Saliva samples are collected before brushing (baseline) and at 30 minutes and 2 hours after brushing to measure bacterial load by change in log CFU/mL. The primary comparison is the change in whole-mouth bacterial counts between the test and control toothbrushes at those post-brushing time points. Participants must be able to attend the site and comply with at-home procedures and sampling.

Who should consider this trial

Good fit: Adults aged 18–65 in good general and oral health with at least 20 permanent natural teeth who can attend study visits and follow at-home procedures.

Not a fit: People with moderate-to-advanced periodontal disease, clinically evident oral tumors, fixed or removable orthodontic appliances, or removable partial dentures were excluded and may not benefit from the results.

Why it matters

Potential benefit: If successful, the 360-degree toothbrush could lower mouth bacteria after brushing and may help reduce short-term plaque buildup and bacterial exposure.

How similar studies have performed: Previous toothbrush-design studies have shown modest differences in plaque and bacterial reduction, but specific clinical data on 360-degree mouth-cleaner designs are limited.

Eligibility criteria

Show full inclusion / exclusion criteria
Inclusion Criteria:

* Provide written, voluntary Informed Consent prior to any study-related procedures.
* Be male or female subjects aged 18 to 65 years, inclusive.
* Be available for the entire duration of the clinical research study, including all scheduled visits and compliance with at-home procedures
* Be in good general health with an appropriate oral health status, based on the medical/dental history and the clinical opinion of the study investigator or dental examiner. -Have a minimum of 20 permanent, natural, uncrowned teeth (excluding third molars)

Exclusion Criteria:

* Current Orthodontic Treatment: Presence of any fixed or removable orthodontic appliances (e.g., braces, aligners with attachments, retainers).
* Prosthetics: Presence of partial removable dentures.
* Oral Pathology: Clinically evident benign or malignant neoplasms (tumors) of the oral mucosa, gingiva, palate, tongue, floor of the mouth, or jaw.
* Periodontal Status: Diagnosis of moderate to advanced periodontitis, defined by periodontal charting (e.g., Clinical Attachment Loss \[CAL\] ≥ 3mm or Probing Depths \[PD\] ≥ 5mm in multiple sites).
* Carious Lesions: Presence of rampant caries (widespread, rapidly progressive lesions) or ≥ 5 active carious lesions (cavitated or non-cavitated) judged by the investigator to require immediate restorative care.
* Recent Antibiotic Use: Use of any systemic antibiotic medication within 30 days prior to the screening or baseline visit.
* Recent Dental Prophylaxis: Received a professional dental cleaning (including hand/ultrasonic scaling and/or polishing) within 14 days prior to the baseline visit.
* Interfering Medications: Current use of prescription medications that, in the investigator's opinion, could interfere with study outcomes by affecting the oral microbiome, salivary flow, or inflammation (e.g., long-term antimicrobials, xerogenic medications).
* Fasting Inability: Inability or unwillingness to comply with the fasting requirement (refraining from all food and drink, other than plain water, for up to 4 hours) required for on-site visits.
* Substance Abuse: Documented history or investigator-assessed ongoing substance misuse that could impact study compliance or subject safety.
* Pregnancy or Lactation: Subjects who are pregnant or currently lactating (breastfeeding), based on self-report at screening.
* Tobacco/Nicotine Use: Any current use of tobacco, nicotine, or e-cigarette products (including combustible cigarettes, smokeless tobacco, vaping, or nicotine replacement therapy).
* Concurrent Study Participation: Participation in any other interventional or observational clinical study within the past 30 days that could confound the results of this trial.

Where this trial is running

Heredia

Study contacts

How to participate

  1. Review the eligibility criteria above with your treating physician.
  2. Visit the official trial page on ClinicalTrials.gov for the most current contact information and recruitment status.
  3. Contact the listed study coordinator or principal investigator to request pre-screening. Pre-screening is free and never obligates you to enroll.
Conditions Bacterial Load Reduction
Last reviewed 2026-06-10 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.