Using CRISPR to improve live cell analysis of stem cells
CRISPR-based genome tagging of iPS Cells for stain-free multicolor live cell analysis
This study is exploring a new way to help find better treatments for diseases by using advanced genetic tools to tag genes in special stem cells, making it easier to see how these cells behave without messy stains, and it's especially aimed at improving research for conditions like cancer.
Quick facts
| Grant type | R21 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Temple Univ of the Commonwealth NIH-funded |
| Lab location | 1 site (Philadelphia, United States) |
| Project ID | NIH-10808178 on NIH RePORTER |
What this research studies
This research focuses on enhancing drug discovery by developing new genetic tools that utilize CRISPR technology to tag genes in induced pluripotent stem cells (iPSCs). By enabling live-cell analysis without the need for traditional staining methods, this approach aims to create more accurate cellular models for testing potential therapeutics. The researchers will employ a patented FAST-HDR vector system to streamline the genome editing process, making it faster and more efficient. This innovative methodology has shown promise in preliminary tests with cancer cell lines and is being adapted for use with iPSCs.
Who could benefit from this research
Good fit: Ideal candidates for this research are individuals who can provide induced pluripotent stem cells, particularly those with specific non-cancer diseases.
Not a fit: Patients with conditions that do not involve cellular models or those who cannot provide iPSCs may not benefit from this research.
Why it matters
Potential benefit: If successful, this research could lead to more effective drug discovery processes, ultimately improving treatment options for patients.
How similar studies have performed: Other research has successfully utilized CRISPR for genome editing in various contexts, indicating a strong potential for success in this novel application.
Where this research is happening
Philadelphia, United States
- Temple Univ of the Commonwealth — Philadelphia, United States (Active)
Researchers
- Principal investigator: Perez, Oscar — Temple Univ of the Commonwealth
- Study coordinator: Perez, Oscar
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.