Using CRISPR technology to study gene function in zebrafish
Official title: CRISPR-Cas13d: Transgenic zebrafish lines toknockdown mRNA
Funded at Stowers Institute for Medical Research · NIH-10752707 · Data updated 2026-11-12
This study is exploring a new way to turn off specific genes in zebrafish to learn more about how genes work and how they relate to diseases, which could help us find better treatments for genetic disorders.
Quick facts
| Grant type | R21 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Stowers Institute for Medical Research NIH-funded |
| Lab location | 1 site (Kansas City, United States) |
| Project start | December 15, 2022 |
| Project end | November 30, 2025 |
| Funding year | 2024 |
| Award (this year) | $247,500 |
| NIH project number | 5R21OD034161-02 |
| Project ID | NIH-10752707 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for this research are individuals with genetic disorders or those interested in the genetic basis of diseases.
✕Less likely to help
Patients with conditions unrelated to genetic mutations or those not involving gene regulation may not benefit from this research.
What this research studies
This research focuses on developing a new method to knock down mRNA in zebrafish using CRISPR-Cas13d technology. By creating transgenic zebrafish lines, the researchers aim to better understand gene functions related to development and diseases. The approach allows for a more systematic investigation of gene regulation without the limitations of traditional gene editing methods. Patients may benefit from insights gained about genetic disorders and potential therapeutic targets.
Why it matters
Potential benefit: If successful, this research could lead to improved understanding and treatment options for genetic disorders.
How similar studies have gone: Other research has successfully utilized CRISPR technology for gene editing, indicating a promising potential for this novel approach.
Where this research is happening
-
Stowers Institute for Medical Research
Kansas City, United States Active
Researchers
- Principal investigator: Bazzini, Ariel — Stowers Institute for Medical Research
- Study coordinator: Bazzini, Ariel
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.