Using advanced genome editing techniques in zebrafish to tag genes.
Epitope Tagging by Prime Editing in Zebrafish
This study is exploring a new way to make precise changes to the DNA of zebrafish, which could help scientists learn more about important processes like how embryos develop and how nerves heal, and it aims to create zebrafish that can use this editing method for future research.
Quick facts
| Grant type | R21 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Temple Univ of the Commonwealth NIH-funded |
| Lab location | 1 site (Philadelphia, United States) |
| Project ID | NIH-10455023 on NIH RePORTER |
What this research studies
This research investigates a new genome editing method called Prime Editing, which allows for precise modifications in the DNA of zebrafish. By utilizing a modified guide RNA and reverse transcriptase, the researchers aim to introduce specific genetic changes that can help in understanding various biological processes, such as embryo development and nerve regeneration. The study will assess the effectiveness of Prime Editors in zebrafish and explore different design variables to optimize this technique. Ultimately, the goal is to create zebrafish lines that can express these editing tools, making them available for broader research applications.
Who could benefit from this research
Good fit: Ideal candidates for this research are individuals interested in genetic research or those with conditions related to genetic mutations that could be modeled in zebrafish.
Not a fit: Patients with conditions that do not have a genetic basis or are not related to the biological processes being studied may not benefit from this research.
Why it matters
Potential benefit: If successful, this research could lead to more accurate genetic modifications in zebrafish, enhancing our understanding of genetic functions and diseases.
How similar studies have performed: Other research has shown promising results with similar genome editing techniques, indicating potential for success in this novel application.
Where this research is happening
Philadelphia, United States
- Temple Univ of the Commonwealth — Philadelphia, United States (Active)
Researchers
- Principal investigator: Balciunas, Darius — Temple Univ of the Commonwealth
- Study coordinator: Balciunas, Darius
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.