Using advanced gene editing to track cell activities over time
Harnessing multiplexed Cas9 genome editing for sequential genetic manipulations and recording activities in cell lineages
This study is working on a new way to use gene editing technology to help scientists track what happens inside cells, especially in areas like cancer and development, so they can better understand how these processes work.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | University of Illinois at Chicago NIH-funded |
| Lab location | 1 site (Chicago, UNITED STATES) |
| Project ID | NIH-10747858 on NIH RePORTER |
What this research studies
This research focuses on developing a new technology that utilizes CRISPR/Cas9 gene editing to create a record of events within a cell's genome. By enabling the tracking of cellular processes in real time, the researchers aim to better understand complex biological phenomena, particularly in cancer and developmental biology. The approach involves creating a series of modular gene editing tools that can be activated in sequence, allowing for detailed lineage tracking of stem cells and the recording of specific signaling events. This innovative method will be validated through next-generation DNA sequencing, providing insights at the individual cell level.
Who could benefit from this research
Good fit: Ideal candidates for this research are individuals with cancers or conditions related to stem cell biology who may benefit from advanced genetic insights.
Not a fit: Patients with stable, non-progressive conditions or those not affected by cancer or stem cell-related issues may not receive benefits from this research.
Why it matters
Potential benefit: If successful, this research could lead to breakthroughs in understanding cancer development and treatment by providing real-time insights into cellular behavior.
How similar studies have performed: Other research has shown promise in using CRISPR technology for genetic manipulation, indicating that this approach could yield significant advancements in understanding cellular processes.
Where this research is happening
Chicago, UNITED STATES
- University of Illinois at Chicago — Chicago, United States (Active)
Researchers
- Principal investigator: Merrill, Bradley J — University of Illinois at Chicago
- Study coordinator: Merrill, Bradley J
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.