Understanding Human Protein Changes with Barcodes
High-throughput biochemistry with RNA-barcoded proteins
This project is creating a new way to quickly understand how changes in human proteins affect their function, which can help us learn more about diseases.
Quick facts
| Grant type | R21 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | University of Washington NIH-funded |
| Lab location | 1 site (Seattle, United States) |
| Project ID | NIH-11118849 on NIH RePORTER |
What this research studies
Many changes in our DNA lead to variations in proteins, but we often don't know how these changes affect our health. This project aims to develop a new method that uses tiny RNA 'barcodes' to quickly test how different protein variations behave. By linking each protein variant to its own barcode, scientists can test many versions at once and use DNA sequencing to read the results. This approach allows proteins to be studied in conditions similar to those inside our bodies, including in human cells, to see how they fold, interact with other molecules, or respond to changes.
Who could benefit from this research
Good fit: This foundational work does not directly involve patient participation but aims to provide insights into human protein variants that are relevant to many different diseases.
Not a fit: Patients seeking immediate clinical interventions or direct treatment options would not directly benefit from this early-stage technology development.
Why it matters
Potential benefit: If successful, this method could help doctors better understand the impact of genetic changes on protein function, leading to more accurate diagnoses and potentially new treatment strategies for various conditions.
How similar studies have performed: This project proposes a novel high-throughput method for characterizing protein variants, building on existing biochemical and sequencing technologies.
Where this research is happening
Seattle, United States
- University of Washington — Seattle, United States (Active)
Researchers
- Principal investigator: Fields, Stanley — University of Washington
- Study coordinator: Fields, Stanley
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.