Understanding how the 3D structure of the genome affects gene expression
Official title: Multimodal Analysis of the Genome Architecture Using Expansion Microscopy
Funded at Yale University · NIH-11046506 · Data updated 2026-11-12
This study is exploring how the way our genes are organized in three dimensions affects how they work, which is important for understanding growth and diseases, and it uses a new technique to look closely at these structures in individual cells.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Yale University NIH-funded |
| Lab location | 1 site (New Haven, United States) |
| Project start | March 1, 2024 |
| Project end | February 29, 2028 |
| Funding year | 2025 |
| Award (this year) | $715,947 |
| NIH project number | 5R01HG012969-02 |
| Project ID | NIH-11046506 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for this research are individuals with genetic conditions or diseases that may be influenced by chromatin architecture and gene expression.
✕Less likely to help
Patients with conditions unrelated to genetic regulation or chromatin structure may not benefit from this research.
What this research studies
This research investigates the three-dimensional organization of the genome and its impact on gene expression, which is crucial for understanding biological processes related to development and disease. The approach involves developing a novel method that combines high-resolution imaging with genomic sequencing to analyze chromatin architecture at the single-cell level. By using expansion microscopy, the researchers aim to visualize the spatial structure of the genome while simultaneously profiling transcription and epigenetic marks. This comprehensive analysis could lead to new insights into how genome structure influences cellular functions.
Why it matters
Potential benefit: If successful, this research could provide groundbreaking insights into gene regulation and potentially lead to new therapeutic strategies for various diseases.
How similar studies have gone: Previous research has shown promise in using advanced imaging techniques to study chromatin structure, but this specific multimodal approach is relatively novel.
Where this research is happening
-
Yale University
New Haven, United States Active
Researchers
- Principal investigator: Giraldez, Antonio J — Yale University
- Study coordinator: Giraldez, Antonio J
Related research
Other NIH-funded projects on the same topics.
-
Aging Well with HIV: Alcohol, Risk Reduction, and Education Center (AWAR3E)
A center at Yale is exploring how aging with HIV interacts with alcohol use to create inflammation and frailty, with the goal of finding biomarkers and potential treatments.
-
Community Health Worker–Led Hypertension Prevention and Control in Nepal
A community health worker–driven approach in Nepal aims to prevent and control high blood pressure among adults by bringing education and care directly to communities and homes.
-
Community health worker-led hypertension prevention and control in Nepal
A community health worker–led program in Nepal aims to help adults prevent and better control high blood pressure through home visits, education, and ongoing support.
-
Adapting a mobile health tool to boost HIV outcomes for older adults with HIV in Ukraine
A mobile health platform called PositiveLinks would be adapted for older adults with HIV in Ukraine to help them stay engaged in care and improve treatment success.
-
Behavioral and neural links between spoken language sounds and written words in young children
This grant studies how young children learn from listening and reading to improve literacy, by looking at how sound patterns transfer to written language.
-
Understanding how autoimmunity causes myasthenia gravis
This project looks at why the immune system attacks the nerve-muscle connection in myasthenia gravis and how different patient groups are affected.
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.