Understanding how proteins bind to DNA in cells
A high-throughput, comprehensive, and quantitative approach for measuring nucleosome-protein binding
This study is looking at how certain proteins that help control genes attach to DNA, which could help us understand important processes in our cells, like how DNA is copied and fixed, and it's designed for anyone interested in the science behind genetics.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | State University of New York at Buffalo NIH-funded |
| Lab location | 1 site (Amherst, United States) |
| Project ID | NIH-10470269 on NIH RePORTER |
What this research studies
This research investigates the mechanisms by which transcription factors, which are proteins that regulate gene expression, bind to DNA within the complex structure of chromatin. By developing a high-throughput next-generation sequencing assay, the study aims to quantitatively analyze thousands of nucleosomes simultaneously, providing insights into how various factors influence protein-DNA interactions. The research will explore aspects such as the orientation of binding sites, the impact of DNA and histone modifications, and cooperative binding dynamics. This comprehensive approach could enhance our understanding of fundamental biological processes like DNA replication and repair.
Who could benefit from this research
Good fit: Ideal candidates for this research are individuals with squamous cell carcinoma or related conditions who may benefit from advancements in understanding gene regulation.
Not a fit: Patients with conditions unrelated to squamous cell carcinoma or those not affected by gene regulation issues may not receive direct benefits from this research.
Why it matters
Potential benefit: If successful, this research could lead to improved strategies for targeting gene regulation in diseases such as cancer.
How similar studies have performed: Other research has shown promising results in understanding protein-DNA interactions, making this approach a continuation of established scientific inquiry.
Where this research is happening
Amherst, United States
- State University of New York at Buffalo — Amherst, United States (Active)
Researchers
- Principal investigator: Buck, Michael Joseph — State University of New York at Buffalo
- Study coordinator: Buck, Michael Joseph
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.