Understanding how heme is inserted into cytochrome P450 enzymes
Official title: Heme Insertase machinery for cytochrome P450 NO synthase
Funded at University of Michigan at Ann Arbor · NIH-11045863 · Data updated 2026-11-12
This study is looking at how certain helper proteins help put a vital part called heme into important enzymes that help our bodies process drugs, which could improve our understanding of how these enzymes work and how they affect our health.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | University of Michigan at Ann Arbor NIH-funded |
| Lab location | 1 site (Ann Arbor, United States) |
| Project start | February 1, 2025 |
| Project end | December 31, 2028 |
| Funding year | 2025 |
| Award (this year) | $343,203 |
| NIH project number | 1R01GM153714-01A1 |
| Project ID | NIH-11045863 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for this research are individuals with conditions that affect drug metabolism or those who are on multiple medications that may interact with cytochrome P450 enzymes.
✕Less likely to help
Patients who do not have any metabolic disorders or are not taking medications that are processed by cytochrome P450 enzymes may not benefit from this research.
What this research studies
This research investigates the process by which heme, an essential component for the function of cytochrome P450 enzymes, is inserted into these proteins. It focuses on the role of specific chaperone proteins, particularly Hsp90 and Hsp70, in facilitating this insertion process. By using a model enzyme called neuronal nitric oxide synthase (nNOS), the research aims to identify the factors involved in heme reconstitution and how they interact within the cell. This could lead to a better understanding of drug metabolism and the biological functions of these enzymes.
Why it matters
Potential benefit: If successful, this research could improve our understanding of drug metabolism and lead to better therapeutic strategies for conditions related to cytochrome P450 dysfunction.
How similar studies have gone: Previous research has shown success in understanding the role of chaperones in protein folding and function, making this approach promising but still novel in the context of heme insertion into cytochrome P450 enzymes.
Where this research is happening
-
University of Michigan at Ann Arbor
Ann Arbor, United States Active
Researchers
- Principal investigator: Osawa, Yoichi — University of Michigan at Ann Arbor
- Study coordinator: Osawa, Yoichi
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.